KRT18 ELISA Kit for Human(Homo sapiens) | EIAab.comKRT18 ELISA Kit for Human(Homo sapiens)

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KRT18 (Gene name),

Keratin, type I cytoskeletal 18 (Protein name ), K1C18_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

KRT18(PIG46;CYK18);

Protein name:

Keratin, type I cytoskeletal 18;

Alternative:

Cytokeratin-18(CK-18);Cell proliferation-inducing gene 46 protein;Keratin-18(K18);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterotetramer of two type I and two type II keratins. Keratin-18 associates with keratin-8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD.

Function:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Subcellular Location:

Cytoplasm perinuclear region

Protein Attributes:

Sequence length:

430

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

430:

SMQTIQKTTT | RRIVDGKVVS | ETNDTKVLRH |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P05783

UniGene:

Hs.406013

Pfam:

PF00038

MIM:

148070

String:

P05783

STRING:

9606.ENSP00000373487

KEGG:

hsa:3875

Uniprot:

P05783

FOR

ELISA Kit for Human Keratin, type I cytoskeletal 18

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E1231m

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96T

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ELISA Kit for Human Keratin, type I cytoskeletal 18

Cat.:

E1231h

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96T

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ELISA Kit for Human Keratin, type I cytoskeletal 18

Cat.:

E1231r

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96T

CLIA Kit for Human Keratin, type I cytoskeletal 18

Cat.:

U1231r

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96T

CLIA Kit for Human Keratin, type I cytoskeletal 18

Cat.:

U1231h

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Manual:

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96T

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CLIA Kit for Human Keratin, type I cytoskeletal 18

Cat.:

U1231m

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96T

Polyclonal Antibody for Human Keratin, type I cytoskeletal 18

Cat.:

P1231Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Keratin, type I cytoskeletal 18

Cat.:

P1231Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human Keratin, type I cytoskeletal 18

Cat.:

P1231Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Keratin, type I cytoskeletal 18

Protein for Human Keratin, type I cytoskeletal 18

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"Comparison of mouse and human keratin 18: a component of intermediate filaments expressed prior to implantation."
Oshima R.G. , Millan J.L. , Cecena G.
Differentiation33:61-68(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY
tissue: Placenta.
2.

"Identification of a cell proliferation-inducing gene."
Kim J.W.
Submitted (2004-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
3.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2004-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
4.

Suzuki Y. , Sugano S. , Totoki Y. , Toyoda A. , Takeda T. , Sakaki Y. , Tanaka A. , Yokoyama S.
Submitted (2005-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Cervix.
tissue: Colon.
tissue: Pancreas.
tissue: Placenta.
tissue: Uterus.
6.

"Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells."
Kulesh D.A. , Oshima R.G.
Mol. Cell. Biol.8:1540-1550(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167
7.

"Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ."
Leube R.E. , Bosch F.X. , Romano V. , Zimbelmann R. , Hofler H. , Franke W.W.
Differentiation33:69-85(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-430;TISSUE SPECIFICITY
tissue: Vulva.
8.

"Cytokeratin expression in simple epithelia. I. Identification of mRNA coding for human cytokeratin no. 18 by a cDNA clone."
Romano V. , Hatzfeld M. , Magin T.M. , Zimbelmann R. , Franke W.W. , Maier G. , Ponstingl H.
Differentiation30:244-253(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 199-430;TISSUE SPECIFICITY
tissue: Liver.
9.

"A two-dimensional gel database of human colon carcinoma proteins."
Ji H. , Reid G.E. , Moritz R.L. , Eddes J.S. , Burgess A.W. , Simpson R.J.
Electrophoresis18:605-613(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE
tissue: Colon carcinoma.
10.

"Characterization and dynamics of O-linked glycosylation of human cytokeratin 8 and 18."
Chou C.F. , Smith A.J. , Omary M.B.
J. Biol. Chem.267:3901-3906(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION
11.

"Identification of the major physiologic phosphorylation site of human keratin 18: potential kinases and a role in filament reorganization."
Ku N.O. , Omary M.B.
J. Cell Biol.127:161-171(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION AT SER-53;MUTAGENESIS OF SER-2; SER-7; SER-10; SER-15; SER-18; SER-23; SER-30; SER-31; SER-34; SER-42; SER-44; SER-47; SER-49; SER-51 AND SER-53
12.

"Identification and mutational analysis of the glycosylation sites of human keratin 18."
Ku N.-O. , Omary M.B.
J. Biol. Chem.270:11820-11827(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT SER-30; SER-31 AND SER-49;MUTAGENESIS OF SER-30; SER-31 AND SER-49;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT SER-2
13.

"Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant."
Ku N.O. , Michie S. , Oshima R.G. , Omary M.B.
J. Cell Biol.131:1303-1314(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF ARG-90
14.

"14-3-3 proteins associate with phosphorylated simple epithelial keratins during cell cycle progression and act as a solubility cofactor."
Liao J. , Omary M.B.
J. Cell Biol.133:345-357(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;INTERACTION WITH YWHAE; YWHAH AND YWHAZ
15.

"Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis."
Caulin C. , Salvesen G.S. , Oshima R.G.
J. Cell Biol.138:1379-1394(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;CLEAVAGE BY CASPASES;MUTAGENESIS OF SER-53 AND ASP-238
16.

"Phosphorylation of human keratin 18 serine 33 regulates binding to 14-3-3 proteins."
Ku N.O. , Liao J. , Omary M.B.
EMBO J.17:1892-1906(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH YWHAE AND YWHAZ;PHOSPHORYLATION AT SER-34;MUTAGENESIS OF SER-34 AND SER-53
17.

"Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus."
Shi J. , Sugrue S.P.
J. Biol. Chem.275:14910-14915(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PNN
18.

"Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein."
Izawa I. , Nishizawa M. , Ohtakara K. , Ohtsuka K. , Inada H. , Inagaki M.
J. Biol. Chem.275:34521-34527(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DNAJB6
19.

"Keratin attenuates tumor necrosis factor-induced cytotoxicity through association with TRADD."
Inada H. , Izawa I. , Nishizawa M. , Fujita E. , Kiyono T. , Takahashi T. , Momoi T. , Inagaki M.
J. Cell Biol.155:415-426(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TRADD
20.

"Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability."
Waseem A. , Karsten U. , Leigh I.M. , Purkis P. , Waseem N.H. , Lane E.B.
Biochemistry43:1283-1295(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ASSOCIATION WITH KRT8
21.

"Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease."
Toivola D.M. , Ku N.O. , Resurreccion E.Z. , Nelson D.R. , Wright T.L. , Omary M.B.
Hepatology40:459-466(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-34 AND SER-53
22.

"Global proteomic approach unmasks involvement of keratins 8 and 18 in the delivery of cystic fibrosis transmembrane conductance regulator (CFTR)/deltaF508-CFTR to the plasma membrane."
Davezac N. , Tondelier D. , Lipecka J. , Fanen P. , Demaugre F. , Debski J. , Dadlez M. , Schrattenholz A. , Cahill M.A. , Edelman A.
Proteomics4:3833-3844(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH MUTATED CFTR;SUBCELLULAR LOCATION
23.

"Identification of trichoplein, a novel keratin filament-binding protein."
Nishizawa M. , Izawa I. , Inoko A. , Hayashi Y. , Nagata K. , Yokoyama T. , Usukura J. , Inagaki M.
J. Cell Sci.118:1081-1090(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TCHP
24.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
25.

"Rescue of DeltaF508-CFTR (cystic fibrosis transmembrane conductance regulator) by curcumin: involvement of the keratin 18 network."
Lipecka J. , Norez C. , Bensalem N. , Baudouin-Legros M. , Planelles G. , Becq F. , Edelman A. , Davezac N.
J. Pharmacol. Exp. Ther.317:500-505(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION AT SER-53
26.

"Interleukin-6 induces keratin expression in intestinal epithelial cells: potential role of keratin-8 in interleukin-6-induced barrier function alterations."
Wang L. , Srinivasan S. , Theiss A.L. , Merlin D. , Sitaraman S.V.
J. Biol. Chem.282:8219-8227(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY;PHOSPHORYLATION;INDUCTION
27.

"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.R. , Zhu Z. , Chan K.C. , Issaq H.J. , Dimitrov D.S. , Veenstra T.D.
J. Proteome Res.6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
28.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
29.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-34; SER-42; SER-60; THR-302 AND SER-399;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
30.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-131 AND LYS-426;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
31.

"O-GlcNAcylation determines the solubility, filament organization, and stability of keratins 8 and 18."
Srikanth B. , Vaidya M.M. , Kalraiya R.D.
J. Biol. Chem.285:34062-34071(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT SER-30; SER-31 AND SER-49
32.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; SER-15; SER-34; SER-42; SER-60; THR-65; SER-100; SER-177; SER-319; SER-399 AND THR-404;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
33.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
34.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
35.

"Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."
Ahmad Y. , Boisvert F.M. , Lundberg E. , Uhlen M. , Lamond A.I.
Mol. Cell. Proteomics11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]
36.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
37.

"Mutation of human keratin 18 in association with cryptogenic cirrhosis."
Ku N.-O. , Wright T.L. , Terrault N.A. , Gish R. , Omary M.B.
J. Clin. Invest.99:19-23(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CIRRH LEU-128
38.

"Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies."
Ku N.-O. , Darling J.M. , Krams S.M. , Esquivel C.O. , Keeffe E.B. , Sibley R.K. , Lee Y.M. , Wright T.L. , Omary M.B.
Proc. Natl. Acad. Sci. U.S.A.100:6063-6068(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CIRRH ALA-103; LEU-128; GLN-261 AND ARG-340;VARIANT THR-230

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