Prkaca ELISA Kit for Mouse(Mus musculus) | EIAab.comPrkaca ELISA Kit for Mouse(Mus musculus)

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Index > Protein center > Prkaca(Gene name) > Mouse

Prkaca (Gene name),

cAMP-dependent protein kinase catalytic subunit alpha (Protein name ), KAPCA_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Prkaca(Pkaca);

Protein name:

cAMP-dependent protein kinase catalytic subunit alpha(PKA C-alpha);

Alternative:

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2.

Function:

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.

Subcellular Location:

Cytoplasm Cell membrane Nucleus Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.

Protein Attributes:

Sequence length:

351

Sequence:

50:

100:

150:

200:

250:

300:

350:

351:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

mmu:18747

UniGene:

Mm.19111

SMR:

P05132

STRING:

10090.ENSMUSP00000005606

Pfam:

PF00069

String:

P05132

Uniprot:

P05132

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ELISA Kit for Mouse PKA C-alpha

Cat.:

E1918h

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96T

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ELISA Kit for Mouse PKA C-alpha

Cat.:

E1918m

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96T

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ELISA Kit for Mouse PKA C-alpha

Cat.:

E1918r

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96T

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ELISA Kit for Mouse PKA C-alpha

Cat.:

E1918d

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96T

ELISA Kit for Mouse PKA C-alpha

Cat.:

E1918p

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96T

ELISA Kit for Mouse PKA C-alpha

Cat.:

E1918b

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96T

CLIA Kit for Mouse PKA C-alpha

Cat.:

U1918m

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Packing:

96T

CLIA Kit for Mouse PKA C-alpha

Cat.:

U1918r

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96T

CLIA Kit for Mouse PKA C-alpha

Cat.:

U1918b

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96T

CLIA Kit for Mouse PKA C-alpha

Cat.:

U1918d

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MSDS:

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Packing:

96T

CLIA Kit for Mouse PKA C-alpha

Cat.:

U1918p

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MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Mouse PKA C-alpha

Cat.:

U1918h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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Polyclonal Antibody for Mouse PKA C-alpha

Cat.:

P1918Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse PKA C-alpha

Cat.:

P1918Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse PKA C-alpha

Cat.:

P1918Rb-m

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40ug/0.2ml

Monoclonal Antibody for Mouse PKA C-alpha

Protein for Mouse PKA C-alpha

Cat.:

R1918h

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Packing:

1mg

Purity:

>90%

Protein for Mouse PKA C-alpha

Cat.:

R1918m

Price:

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Packing:

1mg

Purity:

>90%

Protein for Mouse PKA C-alpha

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Characterization of genomic clones coding for the C alpha and C beta subunits of mouse cAMP-dependent protein kinase."
Chrivia J.C. , Uhler M.D. , McKnight G.S.
J. Biol. Chem.263:5739-5744(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1)
2.

"Isolation of cDNA clones coding for the catalytic subunit of mouse cAMP-dependent protein kinase."
Uhler M.D. , Carmichael D.F. , Lee D.C. , Chrivia J.C. , Krebs E.G. , McKnight G.S.
Proc. Natl. Acad. Sci. U.S.A.83:1300-1304(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain: FVB/N-3.
tissue: Mammary tumor.
4.

"The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative C-alpha mRNA expressed specifically in spermatogenic cells."
San Agustin J.T. , Wilkerson C.G. , Witman G.B.
Mol. Biol. Cell11:3031-3044(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-153 (ISOFORM 2)
tissue: Testis.
5.

"Identification of phosphorylation sites in the recombinant catalytic subunit of cAMP-dependent protein kinase."
Yonemoto W. , Garrod S.M. , Bell S.M. , Taylor S.S.
J. Biol. Chem.268:18626-18632(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-11; SER-140; THR-198 AND SER-339
6.

"Phosphorylation and activation of cAMP-dependent protein kinase by phosphoinositide-dependent protein kinase."
Cheng X. , Ma Y. , Moore M. , Hemmings B.A. , Taylor S.S.
Proc. Natl. Acad. Sci. U.S.A.95:9849-9854(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-198 BY PDPK1
7.

"Influence of myristoylation, phosphorylation, and deamidation on the structural behavior of the N-terminus of the catalytic subunit of cAMP-dependent protein kinase."
Tholey A. , Pipkorn R. , Bossemeyer D. , Kinzel V. , Reed J.
Biochemistry40:225-231(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MYRISTOYLATION AT GLY-2;PHOSPHORYLATION AT SER-11;DEAMIDATION AT ASN-3
8.

"Mutation of the Calpha subunit of PKA leads to growth retardation and sperm dysfunction."
Skaalhegg B.S. , Huang Y. , Su T. , Idzerda R.L. , McKnight G.S. , Burton K.A.
Mol. Endocrinol.16:630-639(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE
9.

"Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility."
Nolan M.A. , Babcock D.F. , Wennemuth G. , Brown W. , Burton K.A. , McKnight G.S.
Proc. Natl. Acad. Sci. U.S.A.101:13483-13488(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN SPERMATOZOA CAPACITATION (ISOFORM 2);TISSUE SPECIFICITY (ISOFORM 2)
10.

"Developmentally acquired PKA localisation in mouse oocytes and embryos."
Webb R.J. , Tinworth L. , Thomas G.M. , Zaccolo M. , Carroll J.
Dev. Biol.317:36-45(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;DEVELOPMENTAL STAGE
11.

"Protein kinase A regulates resumption of meiosis by phosphorylation of Cdc25B in mammalian oocytes."
Pirino G. , Wescott M.P. , Donovan P.J.
Cell Cycle8:665-670(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN MEIOSIS RESUMPTION;FUNCTION AS CDC25B KINASE;INTERACTION WITH CDC25B
12.

"Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa; its implications during capacitation."
Baker M.A. , Hetherington L. , Curry B. , Aitken R.J.
Dev. Biol.333:57-66(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN SPERMATOZOA CAPACITATION;FUNCTION AS ABL1 KINASE;INTERACTION WITH ABL1
13.

"Direct modulation of the protein kinase A catalytic subunit alpha by growth factor receptor tyrosine kinases."
Caldwell G.B. , Howe A.K. , Nickl C.K. , Dostmann W.R. , Ballif B.A. , Deming P.B.
J. Cell. Biochem.113:39-48(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-331
14.

"Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase."
Knighton D.R. , Zheng J. , ten Eyck L.F. , Ashford V.A. , Xuong N.-H. , Taylor S.S. , Sowadski J.M.
Science253:407-414(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS)
15.

"Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor."
Zheng J. , Knighton D.R. , ten Eyck L.F. , Karlsson R. , Xuong N.-H. , Taylor S.S. , Sowadski J.M.
Biochemistry32:2154-2161(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF COMPLEX WITH INHIBITOR
16.

"Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine."
Narayana N. , Cox S. , Shaltiel S. , Taylor S.S. , Xuong N.
Biochemistry36:4438-4448(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF COMPLEX WITH INHIBITOR
17.

"A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility."
Narayana N. , Cox S. , Nguyen-Huu X. , ten Eyck L.F. , Taylor S.S.
Structure5:921-935(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS)
18.

"PKA-I holoenzyme structure reveals a mechanism for cAMP-dependent activation."
Kim C. , Cheng C.Y. , Saldanha S.A. , Taylor S.S.
Cell130:1032-1043(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH ATP ANALOG
19.

"PKA type IIalpha holoenzyme reveals a combinatorial strategy for isoform diversity."
Wu J. , Brown S.H. , von Daake S. , Taylor S.S.
Science318:274-279(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS)
20.

"Contribution of non-catalytic core residues to activity and regulation in protein kinase A."
Yang J. , Kennedy E.J. , Wu J. , Deal M.S. , Pennypacker J. , Ghosh G. , Taylor S.S.
J. Biol. Chem.284:6241-6248(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH ADP;SUBUNIT;MUTAGENESIS OF LYS-286 AND PHE-328
21.

"Novel isoform-specific interfaces revealed by PKA RIIbeta holoenzyme structures."
Brown S.H.J. , Wu J. , Kim C. , Alberto K. , Taylor S.S.
J. Mol. Biol.393:1070-1082(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.62 ANGSTROMS) IN COMPLEX WITH ATP ANALOGS
22.

"Dynamics connect substrate recognition to catalysis in protein kinase A."
Masterson L.R. , Cheng C. , Yu T. , Tonelli M. , Kornev A. , Taylor S.S. , Veglia G.
Nat. Chem. Biol.6:821-828(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH ATP ANALOG AND SUBSTRATE PEPTIDE;ENZYME REGULATION
23.

"Structure and allostery of the PKA RIIbeta tetrameric holoenzyme."
Zhang P. , Smith-Nguyen E.V. , Keshwani M.M. , Deal M.S. , Kornev A.P. , Taylor S.S.
Science335:712-716(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 2-351 IN COMPLEX WITH PRKAR2B;SUBUNIT;PHOSPHORYLATION AT SER-140 AND THR-198

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