Pkm2 ELISA Kit for Mouse(Mus musculus) | EIAab.comPkm2 ELISA Kit for Mouse(Mus musculus)

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Pkm2 (Gene name),

Pyruvate kinase isozymes M1/M2 (Protein name ), KPYM_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Pkm2(Pk3;Pykm;Pykm);

Protein name:

Pyruvate kinase isozymes M1/M2;

Alternative:

Pyruvate kinase muscle isozyme;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Monomer and homotetramer. Exists as a monomer in the absence of fructose 1,6 bi-phosphate (FBP), and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. Interacts with HERC1, POU5F1 and PML.

Function:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

531

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

531:

GFFKKGDVVI | VLTGWRPGSG | FTNTMRVVPV | P

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00224

UniGene:

Mm.326167

String:

P52480

KEGG:

mmu:18746

SMR:

P52480

Uniprot:

P52480

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ELISA Kit for Mouse Pyruvate kinase isozymes M1/M2

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E0588h

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96T

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ELISA Kit for Mouse Pyruvate kinase isozymes M1/M2

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E0588r

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96T

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ELISA Kit for Mouse Pyruvate kinase isozymes M1/M2

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E0588m

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ELISA Kit for Mouse Pyruvate kinase isozymes M1/M2

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ELISA Kit for Mouse Pyruvate kinase isozymes M1/M2

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E0588c

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96T

CLIA Kit for Mouse Pyruvate kinase isozymes M1/M2

Cat.:

U0588r

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96T

CLIA Kit for Mouse Pyruvate kinase isozymes M1/M2

Cat.:

U0588h

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96T

CLIA Kit for Mouse Pyruvate kinase isozymes M1/M2

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CLIA Kit for Mouse Pyruvate kinase isozymes M1/M2

Cat.:

U0588m

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96T

CLIA Kit for Mouse Pyruvate kinase isozymes M1/M2

Cat.:

U0588c

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96T

Polyclonal Antibody for Mouse Pyruvate kinase isozymes M1/M2

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P0588Rb-r

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40ug/0.2ml

Polyclonal Antibody for Mouse Pyruvate kinase isozymes M1/M2

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P0588Rb-h

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Polyclonal Antibody for Mouse Pyruvate kinase isozymes M1/M2

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P0588Rb-m

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Monoclonal Antibody for Mouse Pyruvate kinase isozymes M1/M2

Protein for Mouse Pyruvate kinase isozymes M1/M2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Molecular cloning of the complementary DNA for the mouse pyruvate kinase M-2 gene whose expression is dependent upon cell differentiation."
Izumi S. , Manabe A. , Tomoyasu A. , Kihara-Negishi F. , Ariga H.
Biochim. Biophys. Acta1267:135-138(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M2)
2.

"Gene expression of mouse M1 and M2 pyruvate kinase isoenzymes correlates with differential poly(A) tract extension of their mRNAs during the development of spermatogenesis."
de Luis O. , del Mazo J.
Biochim. Biophys. Acta1396:294-305(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M2)
strain: Swiss.
3.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM M2);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-395 (ISOFORM M1)
strain: C57BL/6J.
strain: NOD.
tissue: Bone marrow.
tissue: Kidney.
tissue: Muellerian duct.
4.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: C57BL/6J.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM M2)
strain: C57BL/6.
strain: FVB/N.
tissue: Brain.
tissue: Mammary tumor.
6.

Lubec G. , Kang S.U. , Klug S. , Yang J.W. , Zigmond M. , Sunyer B. , Chen W.-Q.
Submitted (2009-01) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 33-56; 67-89; 93-115; 126-136; 142-162; 167-224; 231-246; 248-255; 279-305; 320-336; 343-399; 401-433; 468-498 AND 506-526;IDENTIFICATION BY MASS SPECTROMETRY
strain: C57BL/6.
strain: OF1.
tissue: Brain.
tissue: Hippocampus.
7.

"Proteomic identification of proteins conjugated to ISG15 in mouse and human cells."
Giannakopoulos N.V. , Luo J.K. , Papov V. , Zou W. , Lenschow D.J. , Jacobs B.S. , Borden E.C. , Li J. , Virgin H.W. , Zhang D.E.
Biochem. Biophys. Res. Commun.336:496-506(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ISGYLATION
8.

"Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription."
Lee J. , Kim H.K. , Han Y.-M. , Kim J.
Int. J. Biochem. Cell Biol.40:1043-1054(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
9.

"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A. , Carey G.R. , Sunyaev S.R. , Gygi S.P.
J. Proteome Res.7:311-318(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-105 AND TYR-148;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Brain.
10.

"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-62; LYS-166; LYS-270; LYS-322 AND LYS-475;SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-66; LYS-166; LYS-322 AND LYS-498;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic fibroblast.

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