PKM2 ELISA Kit for Rabbit(Oryctolagus cuniculus) | EIAab.comPKM2 ELISA Kit for Rabbit(Oryctolagus cuniculus)

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Index > Protein center > PKM2(Gene name) > Rabbit

PKM2 (Gene name),

Pyruvate kinase isozymes M1/M2 (Protein name ), KPYM_RABIT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

PKM2(PKM2);

Protein name:

Pyruvate kinase isozymes M1/M2;

Alternative:

Pyruvate kinase muscle isozyme;

Organism:

Rabbit (Oryctolagus cuniculus).

General Annotation

Sub Unit:

Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. Interacts with HERC1 POU5F1 and PML.

Function:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

531

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

531:

GFFKKGDVVI | VLTGWRPGSG | FTNTMRVVPV | P

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00224

String:

P11974

UniGene:

Ocu.2156

SMR:

P11974

Uniprot:

P11974

FOR

ELISA Kit for Rabbit Pyruvate kinase isozymes M1/M2

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E0588h

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96T

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ELISA Kit for Rabbit Pyruvate kinase isozymes M1/M2

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E0588r

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96T

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ELISA Kit for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

E0588m

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96T

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ELISA Kit for Rabbit Pyruvate kinase isozymes M1/M2

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ELISA Kit for Rabbit Pyruvate kinase isozymes M1/M2

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E0588c

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96T

CLIA Kit for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

U0588r

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96T

CLIA Kit for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

U0588h

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96T

CLIA Kit for Rabbit Pyruvate kinase isozymes M1/M2

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CLIA Kit for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

U0588m

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96T

CLIA Kit for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

U0588c

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96T

Polyclonal Antibody for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

P0588Rb-r

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40ug/0.2ml

Polyclonal Antibody for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

P0588Rb-h

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Polyclonal Antibody for Rabbit Pyruvate kinase isozymes M1/M2

Cat.:

P0588Rb-m

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40ug/0.2ml

Monoclonal Antibody for Rabbit Pyruvate kinase isozymes M1/M2

Protein for Rabbit Pyruvate kinase isozymes M1/M2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Structure of rabbit muscle pyruvate kinase complexed with Mn2+, K+, and pyruvate."
Larsen T.M. , Laughlin L.T. , Holden H.M. , Rayment I. , Reed G.H.
Biochemistry33:6301-6309(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M1);X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEX WITH MANGANESE AND PAOTASSIUM IONS AND PYRUVATE
tissue: Skeletal muscle.
2.

Laughlin L.T.
Submitted (1997-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 234-235
3.

"Effects of conserved residues on the regulation of rabbit muscle pyruvate kinase."
Cheng X. , Friesen R.H.E. , Lee J.C.
J. Biol. Chem.271:6313-6321(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M1)
4.

"Interfacial communications in recombinant rabbit kidney pyruvate kinase."
Friesen R.H.E. , Chin A.J. , Ledman D.W. , Lee J.C.
Biochemistry37:2949-2960(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M2)
tissue: Kidney.
5.

"Isolation and sequence determination of an active site peptide of rabbit muscle pyruvate kinase."
Bezares G. , Eyzaguirre J. , Hinrichs M.V. , Heinrikson R.L. , Reardon I. , Kemp R.G. , Latshaw S.P. , Bazaes S.
Arch. Biochem. Biophys.253:133-137(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 343-376
6.

"Ligand-induced domain movement in pyruvate kinase: structure of the enzyme from rabbit muscle with Mg2+, K+, and L-phospholactate at 2.7-A resolution."
Larsen T.M. , Benning M.M. , Wesenberg G.E. , Rayment I. , Reed G.H.
Arch. Biochem. Biophys.345:199-206(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH L-PHOSPHOLACTATE;MAGNESIUM AND POTASSIUM IONS
tissue: Skeletal muscle.
7.

"Structure of the bis(Mg2+)-ATP-oxalate complex of the rabbit muscle pyruvate kinase at 2.1 A resolution: ATP binding over a barrel."
Larsen T.M. , Benning M.M. , Rayment I. , Reed G.H.
Biochemistry37:6247-6255(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH OXYLATE AND MAGNESIUM IONS
tissue: Skeletal muscle.

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