Lepr ELISA Kit for Mouse(Mus musculus) | EIAab.comLepr ELISA Kit for Mouse(Mus musculus) | EIAab.com

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Lepr (Gene name),

Leptin receptor (Protein name ), LEPR_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Lepr(Db;Obr);

Protein name:

Leptin receptor(LEP-R);

Alternative:

OB receptor(OB-R);B219;

CD:

CD295;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3. Interaction with SOCS3 inhibits JAK/STAT signaling.

Function:

Isoform E could function as a transport protein.

Subcellular Location:

Isoform E Secreted

Protein Attributes:

Sequence length:

1162

Sequence:

50:

MMCQKFYVVL | LHWEFLYVIA | ALNLAYPISP | WKFKLFCGPP | NTTDDSFLSP |

100:

AGAPNNASAL | KGASEAIVEA | KFNSSGIYVP | ELSKTVFHCC | FGNEQGQNCS |

150:

ALTDNTEGKT | LASVVKASVF | RQLGVNWDIE | CWMKGDLTLF | ICHMEPLPKN |

200:

PFKNYDSKVH | LLYDLPEVID | DSPLPPLKDS | FQTVQCNCSL | RGCECHVPVP |

250:

RAKLNYALLM | YLEITSAGVS | FQSPLMSLQP | MLVVKPDPPL | GLHMEVTDDG |

300:

NLKISWDSQT | MAPFPLQYQV | KYLENSTIVR | EAAEIVSATS | LLVDSVLPGS |

350:

SYEVQVRSKR | LDGSGVWSDW | SSPQVFTTQD | VVYFPPKILT | SVGSNASFHC |

400:

IYKNENQIIS | SKQIVWWRNL | AEKIPEIQYS | IVSDRVSKVT | FSNLKATRPR |

450:

GKFTYDAVYC | CNEQACHHRY | AELYVIDVNI | NISCETDGYL | TKMTCRWSPS |

500:

TIQSLVGSTV | QLRYHRRSLY | CPDSPSIHPT | SEPKNCVLQR | DGFYECVFQP |

550:

IFLLSGYTMW | IRINHSLGSL | DSPPTCVLPD | SVVKPLPPSN | VKAEITVNTG |

600:

LLKVSWEKPV | FPENNLQFQI | RYGLSGKEIQ | WKTHEVFDAK | SKSASLLVSD |

650:

LCAVYVVQVR | CRRLDGLGYW | SNWSSPAYTL | VMDVKVPMRG | PEFWRKMDGD |

700:

VTKKERNVTL | LWKPLTKNDS | LCSVRRYVVK | HRTAHNGTWS | EDVGNRTNLT |

750:

FLWTEPAHTV | TVLAVNSLGA | SLVNFNLTFS | WPMSKVSAVE | SLSAYPLSSS |

800:

CVILSWTLSP | DDYSLLYLVI | EWKILNEDDG | MKWLRIPSNV | KKFYIHDNFI |

850:

PIEKYQFSLY | PVFMEGVGKP | KIINGFTKDA | IDKQQNDAGL | YVIVPIIISS |

900:

CVLLLGTLLI | SHQRMKKLFW | DDVPNPKNCS | WAQGLNFQKP | ETFEHLFTKH |

950:

AESVIFGPLL | LEPEPISEEI | SVDTAWKNKD | EMVPAAMVSL | LLTTPDPESS |

1000:

SICISDQCNS | ANFSGSQSTQ | VTCEDECQRQ | PSVKYATLVS | NDKLVETDEE |

1050:

QGFIHSPVSN | CISSNHSPLR | QSFSSSSWET | EAQTFFLLSD | QQPTMISPQL |

1100:

SFSGLDELLE | LEGSFPEENH | REKSVCYLGV | TSVNRRESGV | LLTGEAGILC |

1150:

TFPAQCLFSD | IRILQERCSH | FVENNLSLGT | SGENFVPYMP | QFQTCSTHSH |

1162:

KIMENKMCDL | TV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

String:

SMR:

UniGene:

Pfam:

Uniprot:

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ELISA Kit for Mouse LEP-R

Cat.:

E0083m

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Packing:

96T

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ELISA Kit for Mouse LEP-R

Cat.:

E0083p

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MSDS:

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Packing:

96T

ELISA Kit for Mouse LEP-R

Cat.:

E0083h

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96T

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ELISA Kit for Mouse LEP-R

Cat.:

E0083r

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MSDS:

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Packing:

96T

CLIA Kit for Mouse LEP-R

Cat.:

U0083r

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MSDS:

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Packing:

96T

CLIA Kit for Mouse LEP-R

Cat.:

U0083p

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MSDS:

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Packing:

96T

CLIA Kit for Mouse LEP-R

Cat.:

U0083h

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96T

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CLIA Kit for Mouse LEP-R

Cat.:

U0083m

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96T

Polyclonal Antibody for Mouse LEP-R

Cat.:

P0083Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse LEP-R

Cat.:

P0083Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse LEP-R

Cat.:

P0083Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse LEP-R

Monoclonal Antibody for Mouse LEP-R

Monoclonal Antibody for Mouse LEP-R

Monoclonal Antibody for Mouse LEP-R

Monoclonal Antibody for Mouse LEP-R

Protein for Mouse LEP-R

Protein for Mouse LEP-R

Protein for Mouse LEP-R

Protein for Mouse LEP-R

R&D Technical Data

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s

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.9%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

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Recovery was determined by spiking various levels of LEPR into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-101
Plasma	98	95-102

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEPR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-99%	95-100%	93-102%	92-103%
Saliva(n=3)	91-103%	93-102%	94-100%	93-101%
heparin plasma(n=5)	95-100%	91-104%	95-100%	94-100%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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References

1.

"Identification and expression cloning of a leptin receptor, OB-R."
Tartaglia L.A. , Dembski M. , Weng X. , Deng N. , Culpepper J. , Devos R. , Richards G.J. , Campfield L.A. , Clark F.T. , Deeds J. , Muir C. , Sanker S. , Moriarty A. , Moore K.J. , Smutko J.S. , Mays G.G. , Woolf E.A. , Monroe C.A. , more...
Cell83:1263-1271(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A)
tissue: Choroid plexus.
2.

"Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice."
Chen H. , Charlat O. , Tartaglia L.A. , Woolf E.A. , Weng X. , Ellis S.J. , Lakey N.D. , Culpepper J. , Moore K.J. , Breitbart R.E. , Duyk G.M. , Tepper R.I. , Morgenstern J.P.
Cell84:491-495(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B)
strain: C57BL/KsJ.
tissue: Hypothalamus.
3.

"Abnormal splicing of the leptin receptor in diabetic mice."
Lee G.-H. , Proenca R. , Montez J.M. , Carroll K.M. , Darvishzadeh J.G. , Lee J.I. , Friedman J.M.
Nature379:632-635(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E)
strain: C57BL/Ks.
tissue: Hypothalamus.
4.

"Novel B219/OB receptor isoforms: possible role of leptin in hematopoiesis and reproduction."
Cioffi J.A. , Shafer A.W. , Zupancic T.J. , Smith-Gbur J. , Mikhail A. , Platika D. , Snodgrass H.R.
Nat. Med.2:585-589(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C)
strain: BALB/c.
tissue: Liver.
5.

"Defective STAT signaling by the leptin receptor in diabetic mice."
Ghilardi N. , Ziegler S. , Wiestner A. , Stoffel R. , Heim M.H. , Skoda R.C.
Proc. Natl. Acad. Sci. U.S.A.93:6231-6235(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B)
strain: FVB/N.
tissue: Spleen.
6.

"Hyperleptinemia, leptin resistance, and polymorphic leptin receptor in the New Zealand obese mouse."
Igel M. , Becker W. , Herberg L. , Joost H.G.
Endocrinology138:4234-4239(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B)
strain: NZO.
tissue: Hypothalamus.
7.

"Fine structure of the murine leptin receptor gene: splice site suppression is required to form two alternatively spliced transcripts."
Chua S.C. , Koutras I.K. , Han L. , Liu S.M. , Kay J. , Young S.J. , Chung W.K. , Leibel R.L.
Genomics45:264-270(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A; B; C AND E)
strain: 129/J.
8.

"Hyperleptinemia and leptin receptor variant Asp600Asn in the obese, hyperinsulinemic KK mouse strain."
Igel M. , Taylor B.A. , Phillips S.J. , Becker W. , Herberg L. , Joost H.G.
J. Endocrinol.21:337-345(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B);VARIANT ASN-600
strain: KK Obese.
tissue: Hypothalamus.
9.

"Murine leptin receptor genomic exon 18b and surrounding sequence."
Banks A.S. , Myers M.G. Jr.
Submitted (1998-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 890-1162 (ISOFORM B)
strain: 129.
10.

"Identification of the Y985 and Y1077 motifs as SOCS3 recruitment sites in the murine leptin receptor."
Eyckerman S. , Broekaert D. , Verhee A. , Vandekerckhove J. , Tavernier J.
FEBS Lett.486:33-37(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-985 AND TYR-1077
11.

"Activation of downstream signals by the long form of the leptin receptor."
Banks A.S. , Davis S.M. , Bates S.H. , Myers M.G. Jr.
J. Biol. Chem.275:14563-14572(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-985 AND TYR-1138;STAT3 ACTIVATION;ERK/FOS ACTIVATION;MUTAGENESIS OF TYR-985; TYR-1077 AND TYR-1138
12.

"SOCS3 mediates feedback inhibition of the leptin receptor via Tyr985."
Bjorbaek C. , Lavery H.J. , Bates S.H. , Olson R.K. , Davis S.M. , Flier J.S. , Myers M.G. Jr.
J. Biol. Chem.275:40649-40657(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SOCS3;MUTAGENESIS OF TYR-985 AND TYR-1138
13.

"Regulation of Jak kinases by intracellular leptin receptor sequences."
Kloek C. , Haq A.K. , Dunn S.L. , Lavery H.J. , Banks A.S. , Myers M.G. Jr.
J. Biol. Chem.277:41547-41555(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN JAK2 ACTIVATION

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