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Index > Protein center > PNLIP(Gene name) > Human
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  • PNLIP (Gene name),
  • Pancreatic triacylglycerol lipase (Protein name ),  LIPP_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    PNLIP;
    Protein name:
    Pancreatic triacylglycerol lipase(PL;PTL;Pancreatic lipase);
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Function:
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    465
    Sequence:
    50:
    MLPLWTLSLL | LGAVAGKEVC | YERLGCFSDD | SPWSGITERP | LHILPWSPKD | 
    100:
    VNTRFLLYTN | ENPNNFQEVA | ADSSSISGSN | FKTNRKTRFI | IHGFIDKGEE | 
    150:
    NWLANVCKNL | FKVESVNCIC | VDWKGGSRTG | YTQASQNIRI | VGAEVAYFVE | 
    200:
    FLQSAFGYSP | SNVHVIGHSL | GAHAAGEAGR | RTNGTIGRIT | GLDPAEPCFQ | 
    250:
    GTPELVRLDP | SDAKFVDVIH | TDGAPIVPNL | GFGMSQVVGH | LDFFPNGGVE | 
    300:
    MPGCKKNILS | QIVDIDGIWE | GTRDFAACNH | LRSYKYYTDS | IVNPDGFAGF | 
    350:
    PCASYNVFTA | NKCFPCPSGG | CPQMGHYADR | YPGKTNDVGQ | KFYLDTGDAS | 
    400:
    NFARWRYKVS | VTLSGKKVTG | HILVSLFGNK | GNSKQYEIFK | GTLKPDSTHS | 
    450:
    NEFDSDVDVG | DLQMVKFIWY | NNVINPTLPR | VGASKIIVET | NVGKQFNFCS | 
    465:
    PETVREEVLL | TLTPC
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    SMR:
    String:
    KEGG:
    Pfam:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human PL
    Cat.:
    E1453r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Human PL
    Cat.:
    E1453h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human PL
    Cat.:
    E1453p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human PL
    MSDS:
    Please sign in first.
    ELISA Kit for Human PL
    Cat.:
    E1453m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human PL
    Cat.:
    U1453m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human PL
    Cat.:
    U1453p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human PL
    Cat.:
    U1453h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human PL
    MSDS:
    Please sign in first.
    CLIA Kit for Human PL
    Cat.:
    U1453r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Human PL
    Cat.:
    P1453Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human PL
    Polyclonal Antibody for Human PL
    Cat.:
    P1453Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human PL
    Cat.:
    P1453Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human PL
    Monoclonal Antibody for Human PL
    Monoclonal Antibody for Human PL
    Monoclonal Antibody for Human PL
    Monoclonal Antibody for Human PL
    Monoclonal Antibody for Human PL
    Protein for Human PL
    Protein for Human PL
    Protein for Human PL
    Protein for Human PL
    Protein for Human PL

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.0%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤9.9%

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    Recovery
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    Recovery was determined by spiking various levels of PNLIP into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    96-104

    Plasma

    101

    97-105

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PNLIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    95-103%

    93-104%

    92-108%

    96-104%

    EDTA plasma(n=5)

    97-105%

    96-106%

    95-106%

    97-106%

    heparin plasma(n=5)

    93-110%

     

    94-107%

    92-110%

    95-108%

     

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    References
    1. 1.
      "Cloning and characterization of human pancreatic lipase cDNA."
      Lowe M.E. , Rosenblum J.L. , Strauss A.W.
      J. Biol. Chem.264:20042-20048(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity."
      Giller T. , Buchwald P. , Blum-Kaelin D. , Hunziker W.
      J. Biol. Chem.267:16509-16516(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Pancreas.
    3. 3.
      "The human pancreatic lipase-encoding gene: structure and conservation of an Alu sequence in the lipase gene family."
      Sims H.F. , Jennens M.L. , Lowe M.E.
      Gene131:281-285(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    4. 4.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P. , Earthrowl M.E. , Grafham D.V. , Rubenfield M. , French L. , Steward C.A. , Sims S.K. , Jones M.C. , Searle S. , Scott C. , Howe K. , Hunt S.E. , Andrews T.D. , Gilbert J.G.R. , Swarbreck D. , Ashurst J.L. , Taylor A. , Battles J. , more...
      Nature429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Lung.
    8. 8.
      "Hydrolysis of retinyl esters by pancreatic triglyceride lipase."
      van Bennekum A.M. , Fisher E.A. , Blaner W.S. , Harrison E.H.
      Biochemistry39:4900-4906(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CATALYTIC ACTIVITY
    9. 9.
      "Structure of human pancreatic lipase."
      Winkler F.K. , D'Arcy A. , Hunziker W.
      Nature343:771-774(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)
    10. 10.
      "Structure of the pancreatic lipase-procolipase complex."
      van Tilbeurgh H. , Sarda L. , Verger R. , Cambillau C.
      Nature359:159-162(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH CLPS
    11. 11.
      "Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography."
      van Tilbeurgh H. , Egloff M.-P. , Martinez C. , Rugani N. , Verger R. , Cambillau C.
      Nature362:814-820(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH CLPS
    12. 12.
      "Structure-function relationships in naturally occurring mutants of pancreatic lipase."
      Carriere F. , Thirstrup K. , Boel E. , Verger R. , Thim L.
      Protein Eng.7:563-569(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 354-465
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