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Index > Protein center > MLANA(Gene name) > Human
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  • MLANA (Gene name),
  • Melanoma antigen recognized by T-cells 1 (Protein name ),  MAR1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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    • Gene name:
    MLANA(MART1);
    Protein name:
    Melanoma antigen recognized by T-cells 1(MART-1);
    Alternative:
    Antigen SK29-AA;Antigen LB39-AA;Protein Melan-A;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with PMEL. Interacts with GPR143.
    Function:
    Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
    Subcellular Location:
    Endoplasmic reticulum membrane Single-pass type III membrane protein Golgi apparatus Golgi apparatus trans-Golgi network membrane Melanosome Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
    Protein Attributes:
    Sequence length:
    118
    Sequence:
    50:
    MPREDAHFIY | GYPKKGHGHS | YTTAEEAAGI | GILTVILGVL | LLIGCWYCRR | 
    100:
    RNGYRALMDK | SLHVGTQCAL | TRRCPQEGFD | HRDSKVSLQE | KNCEPVVPNA | 
    118:
    PPAYEKLSAE | QSPPPYSP
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    Hs.154069
    STRING:
    9606.ENSP00000370880
    String:
    Q16655
    MIM:
    605513
    KEGG:
    hsa:2315
    Uniprot:
    Q16655
     
    FOR
    ELISA Kit for Human MART-1
    Cat.:
    E1097h
    Price:
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    Manual:
    Looking for manual.
    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human MART-1
    Cat.:
    U1097h
    Price:
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    Manual:
    Looking for manual.
    MSDS:
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    Packing:
    96T
    Range:
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    Polyclonal Antibody for Human MART-1
    Cat.:
    P1097Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human MART-1
    Protein for Human MART-1

    R&D Technical Data

          12% SDS-PAGE Analysis

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 8.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 11.5%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-105

    Plasma

    98

    93-107

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1：2

    1:4

    1:8

    1:16

    serum(n=5)

    93-105%

    92-101%

    95-105%

    94-106%

    EDTA plasma(n=5)

    89-97%

    93-102%

    89-101%

    92-101%

    heparin plasma(n=5)

    95-106%

     

    94-102%

    101-107%

    96-108%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor."
      Kawakami Y. , Eliyahu S. , Delgado C.H. , Robbins P.F. , Rivoltini L. , Topalian S.L. , Miki T. , Rosenberg S.A.
      Proc. Natl. Acad. Sci. U.S.A.91:3515-3519(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Melanoma.
    2. 2.
      "A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas."
      Coulie P.G. , Brichard V. , van Pel A. , Woelfel T. , Schneider J. , Traversari C. , Mattei S. , de Plaen E. , Lurquin C. , Szikora J.-P. , Renauld J.-C. , Boon T.
      J. Exp. Med.180:35-42(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Amygdala.
    4. 4.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
      Submitted (2004-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      "DNA sequence and analysis of human chromosome 9."
      Humphray S.J. , Oliver K. , Hunt A.R. , Plumb R.W. , Loveland J.E. , Howe K.L. , Andrews T.D. , Searle S. , Hunt S.E. , Scott C.E. , Jones M.C. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Ashwell R.I.S. , Babbage A.K. , Babbage S. , Bagguley C.L. , more...
      Nature429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Skin.
    8. 8.
      "Subcellular localization of the melanoma-associated protein Melan-AMART-1 influences the processing of its HLA-A2-restricted epitope."
      Rimoldi D. , Muehlethaler K. , Salvi S. , Valmori D. , Romero P. , Cerottini J.C. , Levy F.
      J. Biol. Chem.276:43189-43196(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    9. 9.
      "The melanocytic protein Melan-A/MART-1 has a subcellular localization distinct from typical melanosomal proteins."
      De Maziere A.M. , Muehlethaler K. , van Donselaar E. , Salvi S. , Davoust J. , Cerottini J.-C. , Levy F. , Slot J.W. , Rimoldi D.
      Traffic3:678-693(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    10. 10.
      "MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes."
      Hoashi T. , Watabe H. , Muller J. , Yamaguchi Y. , Vieira W.D. , Hearing V.J.
      J. Biol. Chem.280:14006-14016(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH SILV
    11. 11.
      "The ocular albinism type 1 (OA1) G-protein-coupled receptor functions with MART-1 at early stages of melanogenesis to control melanosome identity and composition."
      Giordano F. , Bonetti C. , Surace E.M. , Marigo V. , Raposo G.
      Hum. Mol. Genet.18:4530-4545(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH GPR143
    12. 12.
      "Structural basis for the presentation of tumor-associated MHC class II-restricted phosphopeptides to CD4+ T cells."
      Li Y. , Depontieu F.R. , Sidney J. , Salay T.M. , Engelhard V.H. , Hunt D.F. , Sette A. , Topalian S.L. , Mariuzza R.A.
      J. Mol. Biol.399:596-603(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 100-114 IN COMPLEX WITH HLA-DR1;PHOSPHORYLATION AT SER-108
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