MBD2 ELISA Kit for Human(Homo sapiens) | EIAab.comMBD2 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > MBD2(Gene name) > Human

MBD2 (Gene name),

Methyl-CpG-binding domain protein 2 (Protein name ), MBD2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

MBD2;

Protein name:

Methyl-CpG-binding domain protein 2;

Alternative:

Methyl-CpG-binding protein MBD2;Demethylase(DMTase);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterodimer with MBD3. Part of the MeCP1 complex that contains HDAC1 and HDAC2. Binds DNMT1, MIZF, GPN1, SIN3A, p66-alpha and p66-beta. Isoform 1 binds DHX9, but isoform 2 does not.

Function:

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemi-methylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Isoform 1 may enhance the activation of some unmethylated cAMP-responsive promoters. Reports about DNA demethylase activity of isoform 2 are contradictory.

Subcellular Location:

Nucleus Nuclear, in discrete foci. Detected at replication foci in late S phase.

Protein Attributes:

Sequence length:

411

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

411:

MDIEMDSGDE | A

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF01429

String:

Q9UBB5

UniGene:

Hs.25674

SMR:

Q9UBB5

MIM:

603547

STRING:

9606.ENSP00000256429

KEGG:

hsa:8932

Uniprot:

Q9UBB5

FOR

ELISA Kit for Human Methyl-CpG-binding domain protein 2

CLIA Kit for Human Methyl-CpG-binding domain protein 2

Polyclonal Antibody for Human Methyl-CpG-binding domain protein 2

Monoclonal Antibody for Human Methyl-CpG-binding domain protein 2

Protein for Human Methyl-CpG-binding domain protein 2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Identification and characterization of a family of mammalian methyl-CpG binding proteins."
Hendrich B. , Bird A.
Mol. Cell. Biol.18:6538-6547(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);NUCLEOTIDE SEQUENCE [MRNA] OF 157-411 (ISOFORM 3);FUNCTION;SUBCELLULAR LOCATION
tissue: Testis.
2.

"Genomic structure and chromosomal mapping of the murine and human mbd1, mbd2, mbd3, and mbd4 genes."
Hendrich B. , Abbott C. , McQueen H. , Chambers D. , Cross S.H. , Bird A.
Mamm. Genome10:906-912(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Lymph.
4.

"A mammalian protein with specific demethylase activity for mCpG DNA."
Bhattacharya S.K. , Ramchandani S. , Cervoni N. , Szyf M.
Nature397:579-583(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION (ISOFORM 1);TISSUE SPECIFICITY
5.

"MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex."
Ng H.-H. , Zhang Y. , Hendrich B. , Johnson C.A. , Turner B.M. , Erdjument-Bromage H. , Tempst P. , Reinberg D. , Bird A.
Nat. Genet.23:58-61(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;IDENTIFICATION BY MASS SPECTROMETRY;INTERACTION WITH MECP1 AND HDAC1
6.

"MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase."
Tatematsu K. , Yamazaki T. , Ishikawa F.
Genes Cells5:677-688(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;HETERODIMERIZATION WITH MBD3;INTERACTION WITH DNMT1
7.

"The minimal repression domain of MBD2b overlaps with the methyl-CpG-binding domain and binds directly to Sin3A."
Boeke J. , Ammerpohl O. , Kegel S. , Moehren U. , Renkawitz R.
J. Biol. Chem.275:34963-34967(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION (ISOFORM 1);INTERACTION WITH SIN3A
8.

"Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1."
Humphrey G.W. , Wang Y. , Russanova V.R. , Hirai T. , Qin J. , Nakatani Y. , Howard B.H.
J. Biol. Chem.276:6817-6824(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HDAC1;IDENTIFICATION BY MASS SPECTROMETRY
9.

"Involvement of a novel zinc finger protein, MIZF, in transcriptional repression by interacting with a methyl-CpG-binding protein, MBD2."
Sekimata M. , Takahashi A. , Murakami-Sekimata A. , Homma Y.
J. Biol. Chem.276:42632-42638(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MIZF
10.

"Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3."
Brackertz M. , Boeke J. , Zhang R. , Renkawitz R.
J. Biol. Chem.277:40958-40966(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH P66ALPHA AND P66BETA;SUBCELLULAR LOCATION
11.

"MBDin, a novel MBD2-interacting protein, relieves MBD2 repression potential and reactivates transcription from methylated promoters."
Lembo F. , Pero R. , Angrisano T. , Vitiello C. , Iuliano R. , Bruni C.B. , Chiariotti L.
Mol. Cell. Biol.23:1656-1665(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GPN1
12.

"Antithetic effects of MBD2a on gene regulation."
Fujita H. , Fujii R. , Aratani S. , Amano T. , Fukamizu A. , Nakajima T.
Mol. Cell. Biol.23:2645-2657(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH DHX9
13.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
14.

"p66alpha and p66beta of the Mi-2/NuRD complex mediate MBD2 and histone interaction."
Brackertz M. , Gong Z. , Leers J. , Renkawitz R.
Nucleic Acids Res.34:397-406(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH GATAD2A AND GATAD2B
15.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181 AND SER-407;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
16.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
17.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
18.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181 AND SER-407;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
20.

"Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA."
Cramer J.M. , Scarsdale J.N. , Walavalkar N.M. , Buchwald W.A. , Ginder G.D. , Williams D.C. Jr.
J. Biol. Chem.289:1294-1302(2014) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DNA-BINDING
21.

"p66Alpha-MBD2 coiled-coil interaction and recruitment of Mi-2 are critical for globin gene silencing by the MBD2-NuRD complex."
Gnanapragasam M.N. , Scarsdale J.N. , Amaya M.L. , Webb H.D. , Desai M.A. , Walavalkar N.M. , Wang S.Z. , Zu Zhu S. , Ginder G.D. , Williams D.C. Jr.
Proc. Natl. Acad. Sci. U.S.A.108:7487-7492(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 360-393 IN COMPLEX WITH GATAD2A;SUBUNIT;INTERACTION WITH GATAD2A

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