Mdc1 ELISA Kit for Mouse(Mus musculus) | EIAab.comMdc1 ELISA Kit for Mouse(Mus musculus)

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Mdc1 (Gene name),

Mediator of DNA damage checkpoint protein 1 (Protein name ), MDC1_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Mdc1(Kiaa0170);

Protein name:

Mediator of DNA damage checkpoint protein 1;

Alternative:

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with CHEK2/CHK2/CDS1, which requires ATM-mediated phosphorylation within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A and TP53BP1. Interacts with ATM and FANCD2, and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of XRCC6/KU70, XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM, interacts with RNF8 (via FHA domain). Interacts with CEP164. When phosphorylated, interacts with APTX (via FHA-like domain) (By similarity). Interacts with H2AFX, which requires phosphorylation of H2AFX. Interacts with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN.

Function:

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

Subcellular Location:

Nucleus Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.

Protein Attributes:

Sequence length:

1707

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1707:

NLEMSST

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q5PSV9

KEGG:

mmu:240087

UniGene:

Mm.218511

Pfam:

PF00498

SMR:

Q5PSV9

Uniprot:

Q5PSV9

FOR

ELISA Kit for Mouse Mediator of DNA damage checkpoint protein 1

CLIA Kit for Mouse Mediator of DNA damage checkpoint protein 1

Polyclonal Antibody for Mouse Mediator of DNA damage checkpoint protein 1

Monoclonal Antibody for Mouse Mediator of DNA damage checkpoint protein 1

Protein for Mouse Mediator of DNA damage checkpoint protein 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Specific association of mouse MDC1/NFBD1 with NBS1 at sites of DNA-damage."
Lee A.C. , Fernandez-Capetillo O. , Pisupati V. , Jackson S.P. , Nussenzweig A.
Cell Cycle4:177-182(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];INTERACTION WITH H2AFX AND NBN;SUBCELLULAR LOCATION
2.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6.
tissue: Brain.
3.

"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N. , Kikuno R. , Ohara R. , Inamoto S. , Koseki H. , Hiraoka S. , Saga Y. , Nagase T. , Ohara O. , Koga H.
DNA Res.10:167-180(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 693-1707
tissue: Embryonic tail.
4.

"Large-scale phosphorylation analysis of mouse liver."
Villen J. , Beausoleil S.A. , Gerber S.A. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168; SER-438; SER-442 AND THR-444;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Liver.
5.

"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M. , English L. , Lemieux S. , Courcelles M. , Desjardins M. , Thibault P.
Immunity30:143-154(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
6.

"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M. , Bailey C.M. , Cunningham D.L. , Heath J.K. , Cooper H.J.
Mol. Cell. Proteomics8:904-912(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic fibroblast.
7.

"HORMAD2 is essential for synapsis surveillance during meiotic prophase via the recruitment of ATR activity."
Kogo H. , Tsutsumi M. , Inagaki H. , Ohye T. , Kiyonari H. , Kurahashi H.
Genes Cells17:897-912(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
8.

"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1496;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic fibroblast.

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