MGMT ELISA Kit for Human(Homo sapiens) | EIAab.comMGMT ELISA Kit for Human(Homo sapiens)

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Index > Protein center > MGMT(Gene name) > Human

MGMT (Gene name),

Methylated-DNA--protein-cysteine methyltransferase (Protein name ), MGMT_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

MGMT;

Protein name:

Methylated-DNA--protein-cysteine methyltransferase;

Alternative:

O-6-methylguanine-DNA-alkyltransferase;6-O-methylguanine-DNA methyltransferase(MGMT);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Function:

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Subcellular Location:

Nucleus

Protein Attributes:

Sequence length:

207

Sequence:

50:

100:

150:

200:

207:

SPPAGRN

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

156569

UniGene:

Hs.501522

STRING:

9606.ENSP00000302111

SMR:

P16455

KEGG:

hsa:4255

Pfam:

PF01035

String:

P16455

Uniprot:

P16455

FOR

ELISA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

E2027h

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96T

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ELISA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

MSDS:

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ELISA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

E2027d

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96T

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ELISA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

E2027r

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96T

ELISA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

E2027m

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96T

CLIA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

U2027r

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96T

CLIA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

U2027m

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Packing:

96T

CLIA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

U2027d

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MSDS:

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Packing:

96T

CLIA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

MSDS:

Please sign in first.

CLIA Kit for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

U2027h

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

P2027Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

P2027Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Methylated-DNA--protein-cysteine methyltransferase

Cat.:

P2027Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Methylated-DNA--protein-cysteine methyltransferase

Monoclonal Antibody for Human Methylated-DNA--protein-cysteine methyltransferase

Protein for Human Methylated-DNA--protein-cysteine methyltransferase

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine."
Tano K. , Shiota S. , Collier J. , Foote R.S. , Mitra S.
Proc. Natl. Acad. Sci. U.S.A.87:686-690(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 1-8
2.

"cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells."
Rydberg B. , Spurr N. , Karran P.
J. Biol. Chem.265:9563-9569(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase."
Koike G. , Maki H. , Takeya H. , Hayakawa H. , Sekiguchi M.
J. Biol. Chem.265:14754-14762(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA."
Hayakawa H. , Koike G. , Sekiguchi M.
J. Mol. Biol.213:739-747(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
6.

"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P. , Earthrowl M.E. , Grafham D.V. , Rubenfield M. , French L. , Steward C.A. , Sims S.K. , Jones M.C. , Searle S. , Scott C. , Howe K. , Hunt S.E. , Andrews T.D. , Gilbert J.G.R. , Swarbreck D. , Ashurst J.L. , Taylor A. , Battles J. , more...
Nature429:375-381(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Placenta.
9.

"Structural and immunological comparison of indigenous human O6-methylguanine-DNA methyltransferase with that encoded by a cloned cDNA."
von Wronski M.A. , Shiota S. , Tano K. , Mitra S. , Bigner D.D. , Brent T.P.
J. Biol. Chem.266:1064-1070(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE;ALKYL GROUP ACCEPTOR
10.

"Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA."
Liem L.-K. , Lim A. , Li B.F.L.
Nucleic Acids Res.22:1613-1619(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
11.

"Mutations in human O6-alkylguanine-DNA alkyltransferase imparting resistance to O6-benzylguanine."
Crone T.M. , Goodtzova K. , Edara S. , Pegg A.E.
Cancer Res.54:6221-6227(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF PRO-138; PRO-140 AND GLY-156
12.

"Alteration of arginine-128 to alanine abolishes the ability of human O6-alkylguanine-DNA alkyltransferase to repair methylated DNA but has no effect on its reaction with O6-benzylguanine."
Kanugula S. , Goodtzova K. , Edara S. , Pegg A.E.
Biochemistry34:7113-7119(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF TYR-114; ARG-128 AND CYS-145
13.

"The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity."
Edara S. , Goodtzova K. , Pegg A.E.
Carcinogenesis16:1637-1642(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF TYR-158
14.

"Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding."
Daniels D.S. , Mol C.D. , Arvai A.S. , Kanugula S. , Pegg A.E. , Tainer J.A.
EMBO J.19:1719-1730(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH DNA AND ZINC IONS;MUTAGENESIS OF ARG-128 AND CYS-145
15.

"Crystal structure of the human O(6)-alkylguanine-DNA alkyltransferase."
Wibley J.E. , Pegg A.E. , Moody P.C.
Nucleic Acids Res.28:393-401(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-176
16.

"The structure of the human AGT protein bound to DNA and its implications for damage detection."
Duguid E.M. , Rice P.A. , He C.
J. Mol. Biol.350:657-666(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-179 IN COMPLEX WITH DNA AND ZINC IONS

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