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Index > Protein center > Mmp3(Gene name) > Rat
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  • Mmp3 (Gene name),
  • Stromelysin-1 (Protein name ),  MMP3_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Mmp3;
    Protein name:
    Stromelysin-1(SL-1);
    Alternative:
    PTR1 protein;Matrix metalloproteinase-3(MMP-3);Transin-1;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    Subcellular Location:
    Secreted extracellular space extracellular matrix
    Protein Attributes:
    Sequence length:
    475
    Sequence:
    50:
    MKGLPVLLWL | CTAVCSSYPL | HGSEEDAGME | VLQKYLENYY | GLEKDVKQFT | 
    100:
    KKKDSSPVVK | KIQEMQKFLG | LKMTGKLDSN | TMELMHKPRC | GVPDVGGFST | 
    150:
    FPGSPKWRKN | HISYRIVNYT | LDLPRESVDS | AIERALKVWE | EVTPLTFSRI | 
    200:
    SEGEADIMIS | FAVEEHGDFI | PFDGPGMVLA | HAYAPGPGTN | GDAHFDDDER | 
    250:
    WTDDVTGTNL | FLVAAHELGH | SLGLFHSANA | EALMYPVYKS | STDLARFHLS | 
    300:
    QDDVDGIQSL | YGPPTESPDV | LVVPTKSNSL | DPETLPMCSS | ALSFDAVSTL | 
    350:
    RGEVLFFKDR | HFWRKSLRTP | EPGFYLISSF | WPSLPSNMDA | AYEVTNRDTV | 
    400:
    FILKGNQIWA | IRGHEELAGY | PKSIHTLGLP | ETVQKIDAAI | SLKDQKKTYF | 
    450:
    FVEDKFWRFD | EKKQSMDPEF | PRKIAENFPG | IGTKVDAVFE | AFGFLYFFSG | 
    475:
    SSQLEFDPNA | GKVTHILKSN | SWFNC
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    SMR:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat SL-1
    Cat.:
    E0101h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat SL-1
    Cat.:
    E0101m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat SL-1
    Cat.:
    E0101r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat SL-1
    MSDS:
    Please sign in first.
    ELISA Kit for Rat SL-1
    Cat.:
    E0101d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat SL-1
    Cat.:
    U0101d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat SL-1
    MSDS:
    Please sign in first.
    CLIA Kit for Rat SL-1
    Cat.:
    U0101r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat SL-1
    Cat.:
    U0101h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat SL-1
    Cat.:
    U0101m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Rat SL-1
    Cat.:
    P0101Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat SL-1
    Polyclonal Antibody for Rat SL-1
    Cat.:
    P0101Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat SL-1
    Polyclonal Antibody for Rat SL-1
    Cat.:
    P0101Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat SL-1
    Monoclonal Antibody for Rat SL-1
    Monoclonal Antibody for Rat SL-1
    Monoclonal Antibody for Rat SL-1
    Monoclonal Antibody for Rat SL-1
    Protein for Rat SL-1
    Protein for Rat SL-1
    Protein for Rat SL-1
    Protein for Rat SL-1
    Protein for Rat SL-1

    R&D Technical Data

    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.3%

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    Recovery

    Recovery was determined by spiking various levels of MMP3 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    96-103

    Plasma

    98

    97-105

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-99%

    93-100%

    95-102%

    98-106%

    EDTA plasma(n=5)

    96-102%

    100-104%

    96-103%

    97-108%

    heparin plasma(n=5)

    98-105%

     

    102-109%

    97-100%

    89-96%

     

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    Citations
    [1].
    Jinchuan Yan, , Xuan Zang, Rui Chen, Wei Yuan, Jie Gong, Cuiping Wang, Ying Li
    References
    1. 1.
      "Epidermal growth factor and oncogenes induce transcription of the same cellular mRNA in rat fibroblasts."
      Matrisian L.M. , Glaichenhaus N. , Gesnel M.-C. , Breathnach R.
      EMBO J.4:1435-1440(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Isolation of the oncogene and epidermal growth factor-induced transin gene: complex control in rat fibroblasts."
      Matrisian L.M. , Gleroy P. , Ruhlmann C. , Gesnel M.-C. , Breathnach R.
      Mol. Cell. Biol.6:1679-1686(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Purification and properties of extracellular matrix-degrading metallo-proteinase overproduced by Rous sarcoma virus-transformed rat liver cell line, and its identification as transin."
      Umenishi F. , Yasumitsu H. , Ashida Y. , Yamauti J. , Umeda M. , Miyazaki K.
      J. Biochem.108:537-543(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-28; 110-119; 309-315 AND 316-325
    4. 4.
      "Structure-function relationships in the collagenase family member transin."
      Sanchez-Lopez R. , Nicholson R. , Gesnel M.-C. , Matrisian L.M. , Breathnach R.
      J. Biol. Chem.263:11892-11899(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS;CHARACTERIZATION
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