Lcn2 ELISA Kit for Mouse(Mus musculus) | EIAab.comLcn2 ELISA Kit for Mouse(Mus musculus)

Search Protein-oriented Product center.

For antibodies, ELISA/CLIA, proteins... Product center

Tips

Index > Protein center > Lcn2(Gene name) > Mouse

Lcn2 (Gene name),

Neutrophil gelatinase-associated lipocalin (Protein name ), NGAL_MOUSE from NCBI database.

top

General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Feedback

Gene name:

Lcn2;

Protein name:

Neutrophil gelatinase-associated lipocalin(NGAL);

Alternative:

SV-40-induced 24P3 protein;Lipocalin-2;p25;p25;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer; disulfide-linked. Heterodimer; disulfide-linked with MMP9.

Function:

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

Subcellular Location:

Secreted Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

Protein Attributes:

Sequence length:

200

Sequence:

50:

100:

150:

200:

3D Structure:

N/A

Predicted Eptitope:

Please Sign in.

Vaild Sequence:

Please Sign in.

Related Databases

Pfam:

PF00061

UniGene:

Mm.9537

SMR:

P11672

KEGG:

mmu:16819

String:

P11672

Uniprot:

P11672

FOR

ELISA Kit for Mouse NGAL

Cat.:

E1388h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

ELISA Kit for Mouse NGAL

Cat.:

E1388r

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

ELISA Kit for Mouse NGAL

Cat.:

E1388m

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

CLIA Kit for Mouse NGAL

Cat.:

U1388r

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Mouse NGAL

Cat.:

U1388h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

CLIA Kit for Mouse NGAL

Cat.:

U1388m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

Polyclonal Antibody for Mouse NGAL

Cat.:

P1388Rb-m

Price:

Please sign in first.

Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse NGAL

Cat.:

P1388Rb-h

Price:

Please sign in first.

Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse NGAL

Cat.:

P1388Rb-r

Price:

Please sign in first.

Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse NGAL

Cat.:

M1388h

Price:

Please sign in first.

Packing:

40ug

Monoclonal Antibody for Mouse NGAL

Protein for Mouse NGAL

Cat.:

R1388h

Price:

Please sign in first.

Packing:

1mg

Purity:

>90%

Protein for Mouse NGAL

Cat.:

R1388m

Price:

Please sign in first.

Packing:

1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	93	87-101
Plasma	90	84-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-105%	99-104%	91-106%	94-108%
heparin plasma(n=5)	101-109%	97-105%	93-99%	91-110%
urine(n=5)	99-103%	97-109%	89-101%	96-108%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism."
Hraba-Renevey S. , Turler H. , Kress M. , Salomon C. , Weil R.
Oncogene4:601-608(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Swiss.
tissue: Kidney.
2.

"An apparent autocrine mechanism amplifies the dexamethasone- and retinoic acid-induced expression of mouse lipocalin-encoding gene 24p3."
Garay-Rojas E. , Harper M. , Hraba-Renevey S. , Kress M.
Gene170:173-180(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6J.
tissue: Bone marrow.
4.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: C57BL/6J.
5.

Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
7.

"Demonstration of a glycoprotein derived from the 24p3 gene in mouse uterine luminal fluid."
Chu S.T. , Huang H.L. , Chen J.M. , Chen Y.H.
Biochem. J.316:545-550(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-200;PARTIAL PROTEIN SEQUENCE;GLYCOSYLATION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY
8.

"The first lipocalin with enzymatic activity."
Peitsch M.C. , Boguski M.S.
Trends Biochem. Sci.16:363-363(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SIMILARITY TO THE LIPOCALIN FAMILY
9.

"Mouse oncogene protein 24p3 is a member of the lipocalin protein family."
Flower D.R. , North A.C.T. , Attwood T.K.
Biochem. Biophys. Res. Commun.180:69-74(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SIMILARITY TO THE LIPOCALIN FAMILY
10.

"An iron delivery pathway mediated by a lipocalin."
Yang J. , Goetz D. , Li J.Y. , Wang W. , Mori K. , Setlik D. , Du T. , Erdjument-Bromage H. , Tempst P. , Strong R. , Barasch J.
Mol. Cell10:1045-1056(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;IRON-BINDING;SIDEROPHORE-BINDING;SUBCELLULAR LOCATION
11.

"Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron."
Flo T.H. , Smith K.D. , Sato S. , Rodriguez D.J. , Holmes M.A. , Strong R.K. , Akira S. , Aderem A.
Nature432:917-921(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;IRON-BINDING;SIDEROPHORE-BINDING;DISRUPTION PHENOTYPE;INDUCTION
12.

"A cell-surface receptor for lipocalin 24p3 selectively mediates apoptosis and iron uptake."
Devireddy L.R. , Gazin C. , Zhu X. , Green M.R.
Cell123:1293-1305(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH SLC22A17;IRON-BINDING
13.

"Lipocalin 2-deficient mice exhibit increased sensitivity to Escherichia coli infection but not to ischemia-reperfusion injury."
Berger T. , Togawa A. , Duncan G.S. , Elia A.J. , You-Ten A. , Wakeham A. , Fong H.E. , Cheung C.C. , Mak T.W.
Proc. Natl. Acad. Sci. U.S.A.103:1834-1839(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE
14.

"A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production."
Devireddy L.R. , Hart D.O. , Goetz D.H. , Green M.R.
Cell141:1006-1017(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;IRON-BINDING;SIDEROPHORE-BINDING
15.

"Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors."
Bandaranayake A.D. , Correnti C. , Ryu B.Y. , Brault M. , Strong R.K. , Rawlings D.J.
Nucleic Acids Res.39:E143-E143(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 21-200;DISULFIDE BOND;GLYCOSYLATION AT ASN-85

Product Feedback Wall

* Email:
*Comments: