Nitric oxide synthase, endothelial (Protein name
), NOS3_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (1)
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Gene name:
NOS3;
Protein name:
Nitric oxide synthase, endothelial;
Alternative:
EC-NOS;Constitutive NOS(cNOS);NOS type III(NOSIII);Endothelial NOS(eNOS);
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Homodimer. Interacts with NOSIP and NOSTRIN.
Function:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Subcellular Location:
Cell membrane
Membrane
caveola
Cytoplasm
cytoskeleton
Golgi apparatus
Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Polyclonal Antibody for Human Nitric oxide synthase, endothelial
Polyclonal Antibody for Human Nitric oxide synthase, endothelial
Monoclonal Antibody for Human Nitric oxide synthase, endothelial
Monoclonal Antibody for Human Nitric oxide synthase, endothelial
Monoclonal Antibody for Human Nitric oxide synthase, endothelial
Monoclonal Antibody for Human Nitric oxide synthase, endothelial
Monoclonal Antibody for Human Nitric oxide synthase, endothelial
Monoclonal Antibody for Human Nitric oxide synthase, endothelial
Protein for Human Nitric oxide synthase, endothelial
Protein for Human Nitric oxide synthase, endothelial
Protein for Human Nitric oxide synthase, endothelial
Protein for Human Nitric oxide synthase, endothelial
Protein for Human Nitric oxide synthase, endothelial
Protein for Human Nitric oxide synthase, endothelial
R&D Technical Data
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
Saliva(n=3)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ENOS13B AND ENOS13C);ALTERNATIVE SPLICING;FUNCTION (ISOFORM ENOS13C);VARIANT ASP-298
8.
Liao J.K.
Submitted (1993-12) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
"The role of phosphatidylinositol-3-oh kinase in platelet-derived nitric oxide release and platelet disaggregation." Zhang Y.
,
Freedman J.E.
Submitted (2001-07) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT ASP-298
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS GLN-112; ASP-298; MET-827; MET-885 AND LEU-982
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT ASP-298
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 411-528 (ISOFORM 1/ENOS13C)
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Cited for: PROTEIN SEQUENCE OF 167-175; 531-540; 835-845; 876-881; 886-898; 1001-1005 AND 1068-1080;TISSUE SPECIFICITY
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Cited for: INTERACTION WITH NOSIP;ENZYME REGULATION;SUBCELLULAR LOCATION
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Cited for: INTERACTION WITH NOSTRIN;ENZYME REGULATION;SUBCELLULAR LOCATION
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Cited for: PHOSPHORYLATION AT SER-114;MUTAGENESIS OF SER-114
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Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 67-480
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Cited for: X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS) OF 66-492
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Cited for: VARIANT SUSCEPTIBILITY TO CORONARY SPASM ASP-298
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Cited for: VARIANT SUSCEPTIBILITY TO CORONARY SPASM ASP-298
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Cited for: VARIANTS [LARGE SCALE ANALYSIS] CYS-474 AND GLN-602
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