Nitric oxide synthase, endothelial (Protein name
), NOS3_PIG from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
NOS3(NOS);
Protein name:
Nitric oxide synthase, endothelial;
Alternative:
EC-NOS;Constitutive NOS(cNOS);NOS type III(NOSIII);Endothelial NOS(eNOS);
Organism:
Pig (Sus scrofa).
General Annotation
Sub Unit:
Homodimer. Interacts with NOSIP and NOSTRIN.
Function:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Subcellular Location:
Membrane
caveola
Cytoplasm
cytoskeleton
Golgi apparatus
Cell membrane
Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Polyclonal Antibody for Pig Nitric oxide synthase, endothelial
Polyclonal Antibody for Pig Nitric oxide synthase, endothelial
Monoclonal Antibody for Pig Nitric oxide synthase, endothelial
Monoclonal Antibody for Pig Nitric oxide synthase, endothelial
Monoclonal Antibody for Pig Nitric oxide synthase, endothelial
Monoclonal Antibody for Pig Nitric oxide synthase, endothelial
Monoclonal Antibody for Pig Nitric oxide synthase, endothelial
Monoclonal Antibody for Pig Nitric oxide synthase, endothelial
Protein for Pig Nitric oxide synthase, endothelial
Protein for Pig Nitric oxide synthase, endothelial
Protein for Pig Nitric oxide synthase, endothelial
Protein for Pig Nitric oxide synthase, endothelial
Protein for Pig Nitric oxide synthase, endothelial
Protein for Pig Nitric oxide synthase, endothelial
R&D Technical Data
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
Saliva(n=3)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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Vaild Sequence:
