Occludin (Protein name
), OCLN_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Gene name:
OCLN;
Protein name:
Occludin;
Alternative:
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Interacts with TJP1/ZO1 and with VAPA.
Function:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Subcellular Location:
Membrane
Multi-pass membrane protein
Cell junction
tight junction
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.7%
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Recovery
Recovery was determined by spiking various levels of OCLN into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
96
91-103
Plasma
97
93-106
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OCLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-102%
90-103%
93-98%
91-100%
EDTA plasma(n=5)
90-106%
93-101%
91-102%
93-102%
heparin plasma(n=5)
93-103%
94-100%
92-100%
91-104%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);DOMAIN;TOPOLOGY
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 4; 5; 6 AND 7);ALTERNATIVE SPLICING
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: INTERACTION WITH TJP1;PHOSPHORYLATION AT TYR-398 AND TYR-402;MUTAGENESIS OF TYR-398 AND TYR-402;SUBCELLULAR LOCATION
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Cited for: PHOSPHORYLATION;DEPHOSPHORYLATION BY PTPRJ;SUBCELLULAR LOCATION
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Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION AT THR-403 AND THR-404;MUTAGENESIS OF THR-404
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321; SER-369 AND SER-370;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 416-522;PHOSPHORYLATION AT SER-490