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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.3%
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Recovery
Recovery was determined by spiking various levels of Pepsin A into serum and plasma .
Sample Type
Average(%)
Recovery Range (%)
Serum
97
94-99
Plasma
96
92-102
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pepsin A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
89-98%
78-92%
86-98%
100-106%
EDTA plasma(n=5)
83-92%
86-99%
91-104%
87-98%
heparin plasma(n=5)
86-101%
89-97%
93-105%
90-102%
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