Pmel ELISA Kit for Mouse(Mus musculus) | EIAab.comPmel ELISA Kit for Mouse(Mus musculus) | EIAab.com

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Pmel (Gene name),

Melanocyte protein PMEL (Protein name ), PMEL_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

Pmel(D10H12S53E;Pmel17;Si;Silv);

Protein name:

Melanocyte protein PMEL;

Alternative:

Premelanosome protein;Melanocyte protein Pmel 17;Silver locus protein;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Heterooligomer; disulfide-linked heterooligomers of M-alpha and M-beta. Interacts with MLANA.

Function:

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures.

Subcellular Location:

M-alpha Secreted

Protein Attributes:

Sequence length:

626

Sequence:

50:

MVGVQRRSFL | PVLVLSALLA | VGALEGSRNQ | DWLGVPRQLV | TKTWNRQLYP |

100:

EWTEVQGSNC | WRGGQVSLRV | INDGPTLVGA | NASFSIALHF | PGSQKVLPDG |

150:

QVIWANNTII | NGSQVWGGQP | VYPQEPDDAC | VFPDGGPCPS | GPKPPKRSFV |

200:

YVWKTWGKYW | QVLGGPVSRS | SIATRHAKLG | THTMEVTVYH | RRGSQSYVPL |

250:

AHASSTFTIT | DQVPFSVSVS | QLQALDGETK | HFLRNHPLIF | ALQLHDPSGY |

300:

LAEADLSYTW | DFGDGTGTLI | SRALDVTHTY | LESGSVTAQV | VLQAAIPLVS |

350:

CGSSPVPGTT | DGYMPTAEAP | GTTSRQGTTT | KVVGTTPGQM | PTTQPSGTTV |

400:

VQMPTTEVTA | TTSEQMLTSA | VIDTTLAEVS | TTEGTGTTPT | RPSGTTVAQA |

450:

TTTEGPDASP | LLPTQSSTGS | ISPLLDDTDT | IMLVKRQVPL | DCVLYRYGSF |

500:

SLALDIVQGI | ESAEILQAVP | FSEGDAFELT | VSCQGGLPKE | ACMDISSPGC |

550:

QPPAQRLCQS | VPPSPDCQLV | LHQVLKGGSG | TYCLNVSLAD | ANSLAVASTQ |

600:

LVVPGQDGGL | GQAPLLVGIL | LVLVAVVLAS | LILGIDLRSR | AQFPKCHMVA |

626:

LTAAPASGLR | ARGLGENSPL | LSGQQV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Pfam:

SMR:

String:

Uniprot:

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ELISA Kit for Mouse Melanocyte protein PMEL

Cat.:

E2299m

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Mouse Melanocyte protein PMEL

Cat.:

E2299h

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Mouse Melanocyte protein PMEL

Cat.:

E2299c

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MSDS:

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Packing:

96T

ELISA Kit for Mouse Melanocyte protein PMEL

Cat.:

E2299b

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MSDS:

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Packing:

96T

CLIA Kit for Mouse Melanocyte protein PMEL

Cat.:

U2299c

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Mouse Melanocyte protein PMEL

Cat.:

U2299m

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Mouse Melanocyte protein PMEL

Cat.:

U2299h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Mouse Melanocyte protein PMEL

Cat.:

U2299b

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MSDS:

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Packing:

96T

Polyclonal Antibody for Mouse Melanocyte protein PMEL

Polyclonal Antibody for Mouse Melanocyte protein PMEL

Cat.:

P2299Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse Melanocyte protein PMEL

Cat.:

P2299Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse Melanocyte protein PMEL

Monoclonal Antibody for Mouse Melanocyte protein PMEL

Monoclonal Antibody for Mouse Melanocyte protein PMEL

Monoclonal Antibody for Mouse Melanocyte protein PMEL

Monoclonal Antibody for Mouse Melanocyte protein PMEL

Protein for Mouse Melanocyte protein PMEL

Protein for Mouse Melanocyte protein PMEL

Protein for Mouse Melanocyte protein PMEL

Protein for Mouse Melanocyte protein PMEL

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.1%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

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Recovery was determined by spiking various levels of PMEL into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	108	105-113
Plasma	107	104-112

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PMEL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-110%	102-113%	100-116%	104-114%
EDTA plasma(n=5)	105-110%	98-116%	104-112%	102-112%
heparin plasma(n=5)	100-114%	103-112%	100-115%	101-113%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Mouse silver mutation is caused by a single base insertion in the putative cytoplasmic domain of Pmel 17."
Kwon B.S. , Halaban R. , Ponnazhagan S. , Kim K. , Chintamaneni C. , Bennett D. , Pickard R.T.
Nucleic Acids Res.23:154-158(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS SILVER
strain: C57BL/6.
tissue: Skin.
2.

"Pmel17 initiates premelanosome morphogenesis within multivesicular bodies."
Berson J.F. , Harper D.C. , Tenza D. , Raposo G. , Marks M.S.
Mol. Biol. Cell12:3451-3464(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEOLYTIC PROCESSING

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