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Index > Protein center > PRTN3(Gene name) > Human
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  • PRTN3 (Gene name),
  • Myeloblastin (Protein name ),  PRTN3_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    PRTN3(MBN);
    Protein name:
    Myeloblastin;
    Alternative:
    C-ANCA antigen;AGP7;Neutrophil proteinase 4(NP-4);Leukocyte proteinase 3(PR-3;PR3);Wegener autoantigen;P29;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    256
    Sequence:
    50:
    MAHRPPSPAL | ASVLLALLLS | GAARAAEIVG | GHEAQPHSRP | YMASLQMRGN | 
    100:
    PGSHFCGGTL | IHPSFVLTAA | HCLRDIPQRL | VNVVLGAHNV | RTQEPTQQHF | 
    150:
    SVAQVFLNNY | DAENKLNDVL | LIQLSSPANL | SASVATVQLP | QQDQPVPHGT | 
    200:
    QCLAMGWGRV | GAHDPPAQVL | QELNVTVVTF | FCRPHNICTF | VPRRKAGICF | 
    250:
    GDSGGPLICD | GIIQGIDSFV | IWGCATRLFP | DFFTRVALYV | DWIRSTLRRV | 
    256:
    EAKGRP
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    SMR:
    String:
    UniGene:
    KEGG:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Myeloblastin
    Cat.:
    E8936h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Myeloblastin
    Cat.:
    E8936m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Myeloblastin
    Cat.:
    U8936m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Myeloblastin
    Cat.:
    U8936h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Myeloblastin
    Cat.:
    P8936Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Myeloblastin
    Cat.:
    P8936Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Myeloblastin
    Monoclonal Antibody for Human Myeloblastin
    Protein for Human Myeloblastin
    Protein for Human Myeloblastin

    R&D Technical Data

    s

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.4%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤9.8%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    102

    91-105

    Plasma

    101

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRNT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    97-105%

    95-108%

    96-106%

    98-104%

    EDTA plasma(n=5)

    95-107%

    96-105%

    97-104%

    95-106%

    heparin plasma(n=5)

    93-110%

     

    94-108%

    94-107%

    92-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Wegener autoantigen and myeloblastin are encoded by a single mRNA."
      Labbaye C. , Musette P. , Cayre Y.E.
      Proc. Natl. Acad. Sci. U.S.A.88:9253-9256(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS ILE-119; THR-135 AND SER-136
    2. 2.
      "The DNA sequence and biology of human chromosome 19."
      Grimwood J. , Gordon L.A. , Olsen A.S. , Terry A. , Schmutz J. , Lamerdin J.E. , Hellsten U. , Goodstein D. , Couronne O. , Tran-Gyamfi M. , Aerts A. , Altherr M. , Ashworth L. , Bajorek E. , Black S. , Branscomb E. , Caenepeel S. , Carrano A.V. , more...
      Nature428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    3. 3.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT ILE-119
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT ILE-119
    5. 5.
      "Three human elastase-like genes coordinately expressed in the myelomonocyte lineage are organized as a single genetic locus on 19pter."
      Zimmer M. , Medcalf R.L. , Fink T.M. , Mattmann C. , Lichter P. , Jenne D.E.
      Proc. Natl. Acad. Sci. U.S.A.89:8215-8219(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20 AND 22-256
    6. 6.
      "Structure, chromosomal assignment, and expression of the gene for proteinase-3. The Wegener's granulomatosis autoantigen."
      Sturrock A.B. , Franklin K.F. , Rao G. , Marshall B.C. , Rebentisch M.B. , Lemons R.S. , Hoidal J.R.
      J. Biol. Chem.267:21193-21199(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-254;VARIANTS THR-135 AND SER-136
    7. 7.
      "Donor-recipient polymorphism of the proteinase 3 gene: a potential target for T-cell alloresponses to myeloid leukemia."
      Clave E. , Molldrem J. , Hensel N. , Raptis A. , Barrett A.J.
      J. Immunother.22:1-6(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-200;VARIANT ILE-119
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-42
    9. 9.
      "Cloning of cDNA for proteinase 3: a serine protease, antibiotic, and autoantigen from human neutrophils."
      Campanelli D. , Melchior M. , Fu Y. , Nakata M. , Shuman H. , Nathan C. , Gabay J.E.
      J. Exp. Med.172:1709-1715(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-256;PROTEIN SEQUENCE OF 28-71; 156-181 AND 196-219
    10. 10.
      "Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells."
      Bories D. , Raynal M.-C. , Solomon D.H. , Darzynkiewicz Z. , Cayre Y.E.
      Cell59:959-968(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-256;VARIANTS ILE-119; THR-135 AND SER-136
    11. 11.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-256;PROTEIN SEQUENCE OF 28-48;VARIANTS ILE-119; THR-135 AND SER-136;IDENTITY OF WEGENER'S AUTOANTIGEN WITH PR-3
    12. 12.
      "Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Structural and functional properties."
      Rao N.V. , Wehner N.G. , Marshall B.C. , Gray W.R. , Gray B.H. , Hoidal J.R.
      J. Biol. Chem.266:9540-9548(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-67 AND 228-244
    13. 13.
      "Characterization of two azurphil granule proteases with active-site homology to neutrophil elastase."
      Wilde C.G. , Snable J.L. , Griffith J.E. , Scott R.W.
      J. Biol. Chem.265:2038-2041(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-47 AND 196-219
    14. 14.
      "Monoclonal antibodies specific for neutrophil proteinase 4. Production and use for isolation of the enzyme."
      Ohlsson K. , Linder C. , Rosengren M.
      Biol. Chem. Hoppe-Seyler371:549-555(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-52
    15. 15.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-48
    16. 16.
      "Wegener's granulomatosis autoantigen is a novel neutrophil serine proteinase."
      Niles J.L. , McCluskey R.T. , Ahmad M.F. , Arnaout M.A.
      Blood74:1888-1893(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-47
    17. 17.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-47
    18. 18.
      "Use of proteinase 3 purified by reverse phase HPLC to detect autoantibodies in systemic vasculitis."
      Gaskin G. , Kendal H. , Coulthart A. , Turner N. , Pusey C.D.
      J. Immunol. Methods180:25-33(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-47;CATALYTIC ACTIVITY
      tissue: Neutrophil.
    19. 19.
      "Anti-neutrophil cytoplasm antibodies in Wegener's granulomatosis recognize an elastinolytic enzyme."
      Ludemann J. , Utecht B. , Gross W.L.
      J. Exp. Med.171:357-362(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-43
    20. 20.
      "A novel form of dipeptidylpeptidase IV found in human serum. Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26)."
      Duke-Cohan J.S. , Morimoto C. , Rocker J.A. , Schlossman S.F.
      J. Biol. Chem.270:14107-14114(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 110-121;VARIANT ILE-119
      tissue: Serum.
    21. 21.
      "Identity of Wegener's autoantigen (p29) with proteinase 3 and myeloblastin."
      Gupta S.K. , Niles J.L. , McCluskey R.T. , Arnaout M.A.
      Blood76:2162-2162(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTITY OF WEGENER'S AUTOANTIGEN WITH PROTEINASE 3
    22. 22.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    23. 23.
      "The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies."
      Fujinaga M. , Charnaia M.M. , Halenbeck R. , Koths K. , James M.N.G.
      J. Mol. Biol.261:267-278(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS)
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