S100A2 ELISA Kit for Human(Homo sapiens) | EIAab.comS100A2 ELISA Kit for Human(Homo sapiens)

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S100A2 (Gene name),

Protein S100-A2 (Protein name ), S10A2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

S100A2(S100L);

Protein name:

Protein S100-A2;

Alternative:

Protein S-100L;CAN19;S100 calcium-binding protein A2;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homomultimeric (Probable). Interacts with FKBP4.

Function:

May act as a modulator against excess calcium accumulation in normal human mammary epithelial cells. May also play a role in suppressing tumor cell growth.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

Sequence:

50:

98:

VDEEGLKKLM | GSLDENSDQQ | VDFQEYAVFL | ALITVMCNDF | FQGCPDRP

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P29034

KEGG:

hsa:6273

MIM:

176993

String:

P29034

UniGene:

Hs.516484

Pfam:

PF01023

STRING:

9606.ENSP00000357697

Uniprot:

P29034

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ELISA Kit for Human Protein S100-A2

Cat.:

E9465b

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Packing:

96T

ELISA Kit for Human Protein S100-A2

Cat.:

E9465h

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Manual:

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Packing:

96T

Range:

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CLIA Kit for Human Protein S100-A2

Cat.:

U9465h

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Manual:

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Packing:

96T

Range:

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CLIA Kit for Human Protein S100-A2

Cat.:

U9465b

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Packing:

96T

Polyclonal Antibody for Human Protein S100-A2

Cat.:

P9465Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Protein S100-A2

Monoclonal Antibody for Human Protein S100-A2

Protein for Human Protein S100-A2

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Recovery was determined by spiking various levels of S10A2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	90-102
Plasma	97	91-103

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S10A2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-104%	91-100%	93-101%	89-103%
EDTA plasma(n=5)	92-102%	90-105%	90-106%	90-106%
heparin plasma(n=5)	93-100%	93-102%	92-103%	88-107%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Down-regulation of a member of the S100 gene family in mammary carcinoma cells and reexpression by azadeoxycytidine treatment."
Lee S.W. , Tomasetto C. , Swisshelm K. , Keyomarsi K. , Sager R.
Proc. Natl. Acad. Sci. U.S.A.89:2504-2508(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;TISSUE SPECIFICITY;INDUCTION
tissue: Mammary epithelium.
2.

"Repression of the candidate tumor suppressor gene S100A2 in breast cancer is mediated by site-specific hypermethylation."
Wicki R. , Franz C. , Scholl F.A. , Heizmann C.W. , Schaefer B.W.
Cell Calcium22:243-254(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];DEVELOPMENTAL STAGE;INDUCTION
3.

"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Ovary.
5.

"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
Rasmussen H.H. , van Damme J. , Puype M. , Gesser B. , Celis J.E. , Vandekerckhove J.
Electrophoresis13:960-969(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 37-40 AND 42-49
tissue: Keratinocyte.
6.

"S100 proteins regulate the interaction of Hsp90 with cyclophilin 40 and FKBP52 through their tetratricopeptide repeats."
Shimamoto S. , Kubota Y. , Tokumitsu H. , Kobayashi R.
FEBS Lett.584:1119-1125(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FKBP4
7.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
8.

"S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation."
Yamaguchi F. , Umeda Y. , Shimamoto S. , Tsuchiya M. , Tokumitsu H. , Tokuda M. , Kobayashi R.
J. Biol. Chem.287:13787-13798(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CALCIUM SIGNALING;INTERACTION WITH PPP5C
9.

"Crystal structure of Ca2+ -free S100A2 at 1.6-A resolution."
Koch M. , Diez J. , Fritz G.
J. Mol. Biol.378:933-942(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS);SUBUNIT

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