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Index > Protein center > AHCY(Gene name) > Human
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  • AHCY (Gene name),
  • Adenosylhomocysteinase (Protein name ),  SAHH_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    AHCY(SAHH);
    Protein name:
    Adenosylhomocysteinase(AdoHcyase);
    Alternative:
    S-adenosyl-L-homocysteine hydrolase;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homotetramer.
    Function:
    Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
    Subcellular Location:
    Cytoplasm Melanosome Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    Protein Attributes:
    Sequence length:
    432
    Sequence:
    50:
    MSDKLPYKVA | DIGLAAWGRK | ALDIAENEMP | GLMRMRERYS | ASKPLKGARI | 
    100:
    AGCLHMTVET | AVLIETLVTL | GAEVQWSSCN | IFSTQDHAAA | AIAKAGIPVY | 
    150:
    AWKGETDEEY | LWCIEQTLYF | KDGPLNMILD | DGGDLTNLIH | TKYPQLLPGI | 
    200:
    RGISEETTTG | VHNLYKMMAN | GILKVPAINV | NDSVTKSKFD | NLYGCRESLI | 
    250:
    DGIKRATDVM | IAGKVAVVAG | YGDVGKGCAQ | ALRGFGARVI | ITEIDPINAL | 
    300:
    QAAMEGYEVT | TMDEACQEGN | IFVTTTGCID | IILGRHFEQM | KDDAIVCNIG | 
    350:
    HFDVEIDVKW | LNENAVEKVN | IKPQVDRYRL | KNGRRIILLA | EGRLVNLGCA | 
    400:
    MGHPSFVMSN | SFTNQVMAQI | ELWTHPDKYP | VGVHFLPKKL | DEAVAEAHLG | 
    432:
    KLNVKLTKLT | EKQAQYLGMS | CDGPFKPDHY | RY
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    MIM:
    String:
    SMR:
    UniGene:
    Pfam:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Human AdoHcyase
    Cat.:
    E12634h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human AdoHcyase
    Cat.:
    E12634m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human AdoHcyase
    MSDS:
    Please sign in first.
    ELISA Kit for Human AdoHcyase
    MSDS:
    Please sign in first.
    ELISA Kit for Human AdoHcyase
    Cat.:
    E12634r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human AdoHcyase
    Cat.:
    U12634r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human AdoHcyase
    MSDS:
    Please sign in first.
    CLIA Kit for Human AdoHcyase
    MSDS:
    Please sign in first.
    CLIA Kit for Human AdoHcyase
    Cat.:
    U12634h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human AdoHcyase
    Cat.:
    U12634m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human AdoHcyase
    Polyclonal Antibody for Human AdoHcyase
    Polyclonal Antibody for Human AdoHcyase
    Cat.:
    P12634Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human AdoHcyase
    Polyclonal Antibody for Human AdoHcyase
    Monoclonal Antibody for Human AdoHcyase
    Monoclonal Antibody for Human AdoHcyase
    Monoclonal Antibody for Human AdoHcyase
    Monoclonal Antibody for Human AdoHcyase
    Monoclonal Antibody for Human AdoHcyase
    Protein for Human AdoHcyase
    Protein for Human AdoHcyase
    Protein for Human AdoHcyase
    Protein for Human AdoHcyase
    Protein for Human AdoHcyase

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11.7%

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    Recovery
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    Recovery was determined by spiking various levels of SAHH into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    108

    105-113

    Plasma

    106

    102-108

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    106-112%

    102-112%

    104-110%

    103-114%

    EDTA plasma(n=5)

    103-114%

    104-114%

    100-115%

    105-111%

    heparin plasma(n=5)

    102-103%

     

    103-112%

    101-114%

    106-109%

     

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    References
    1. 1.
      "Sequence of full length cDNA for human S-adenosylhomocysteine hydrolase."
      Coulter-Karis D.E. , Hershfield M.S.
      Ann. Hum. Genet.53:169-175(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);VARIANT ASN-86
      tissue: Placenta.
    2. 2.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2004-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    3. 3.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
      tissue: Testis.
      tissue: Umbilical cord blood.
    4. 4.
      "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P. , Matthews L.H. , Ashurst J.L. , Burton J. , Gilbert J.G.R. , Jones M. , Stavrides G. , Almeida J.P. , Babbage A.K. , Bagguley C.L. , Bailey J. , Barlow K.F. , Bates K.N. , Beard L.M. , Beare D.M. , Beasley O.P. , Bird C.P. , Blakey S.E. , more...
      Nature414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Placenta.
      tissue: Skin.
    7. 7.
      "Limited proteolysis of S-adenosylhomocysteine hydrolase: implications for the three-dimensional structure."
      Gupta R.A. , Yuan C.-S. , Ault-Riche D.B. , Borchardt R.T.
      Arch. Biochem. Biophys.319:365-371(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-6; 104-108 AND 198-202
    8. 8.
      Bienvenut W.V. , Heiserich L. , Gottlieb E.
      Submitted (2008-03) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-34; 95-103; 143-186; 215-226; 336-343 AND 389-405;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: Colon carcinoma.
    9. 9.
      "Isozyme and DNA analysis of human S-adenosyl-L-homocysteine hydrolase (AHCY)."
      Arredondo-Vega F.X. , Charlton J.A. , Edwards Y.H. , Hopkinson D.A. , Whitehouse D.B.
      Ann. Hum. Genet.53:157-167(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-432 (ISOFORM 1)
      tissue: Placenta.
    10. 10.
      "Photoaffinity labeling of human placental S-adenosylhomocysteine hydrolase with [2-3H]8-azido-adenosine."
      Yuan C.S. , Borchardt R.T.
      J. Biol. Chem.270:16140-16146(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 175-186 AND 319-327
    11. 11.
      "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
      Chi A. , Valencia J.C. , Hu Z.-Z. , Watabe H. , Yamaguchi H. , Mangini N.J. , Huang H. , Canfield V.A. , Cheng K.C. , Yang F. , Abe R. , Yamagishi S. , Shabanowitz J. , Hearing V.J. , Wu C. , Appella E. , Hunt D.F.
      J. Proteome Res.5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]
      tissue: Melanoma.
    12. 12.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    13. 13.
      "Structure determination of selenomethionyl S-adenosylhomocysteine hydrolase using data at a single wavelength."
      Turner M.A. , Yuan C.S. , Borchardt R.T. , Hershfield M.S. , Smith G.D. , Howell P.L.
      Nat. Struct. Biol.5:369-376(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NAD AND ADENOSINE ANALOG;SUBUNIT;COFACTOR
      tissue: Placenta.
    14. 14.
      "Catalytic strategy of S-adenosyl-L-homocysteine hydrolase: transition-state stabilization and the avoidance of abortive reactions."
      Yang X. , Hu Y. , Yin D.H. , Turner M.A. , Wang M. , Borchardt R.T. , Howell P.L. , Kuczera K. , Schowen R.L.
      Biochemistry42:1900-1909(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) IN COMPLEX WITH NAD AND NEPLANOCIN A;FUNCTION;COFACTOR
    15. 15.
      "Effect of genetic variation in the human S-adenosylhomocysteine hydrolase gene on total homocysteine concentrations and risk of recurrent venous thrombosis."
      Gellekink H. , den Heijer M. , Kluijtmans L.A. , Blom H.J.
      Eur. J. Hum. Genet.12:942-948(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT TRP-38
    16. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT HMAHCHD CYS-143
    17. 17.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS HMAHCHD VAL-89 AND CYS-143
    18. 18.
      "S-adenosylhomocysteine hydrolase (AHCY) deficiency: two novel mutations with lethal outcome."
      Vugrek O. , Beluzic R. , Nakic N. , Mudd S.H.
      Hum. Mutat.30:E555-E565(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS HMAHCHD CYS-49 AND GLY-86
    19. 19.
      "S-adenosylhomocysteine hydrolase deficiency: two siblings with fetal hydrops and fatal outcomes."
      Grubbs R. , Vugrek O. , Deisch J. , Wagner C. , Stabler S. , Allen R. , Baric I. , Rados M. , Mudd S.H.
      J. Inherit. Metab. Dis.33:705-713(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS HMAHCHD CYS-49 AND GLY-86
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