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Index > Protein center > Ahcy(Gene name) > Mouse
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  • Ahcy (Gene name),
  • Adenosylhomocysteinase (Protein name ),  SAHH_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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    • Gene name:
    Ahcy;
    Protein name:
    Adenosylhomocysteinase(AdoHcyase);
    Alternative:
    Liver copper-binding protein;CUBP;S-adenosyl-L-homocysteine hydrolase;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Homotetramer.
    Function:
    Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
    Subcellular Location:
    Cytoplasm Melanosome
    Protein Attributes:
    Sequence length:
    432
    Sequence:
    50:
    MSDKLPYKVA | DIGLAAWGRK | ALDIAENEMP | GLMRMREMYS | ASKPLKGARI | 
    100:
    AGCLHMTVET | AVLIETLVAL | GAEVRWSSCN | IFSTQDHAAA | AIAKAGIPVF | 
    150:
    AWKGETDEEY | LWCIEQTLHF | KDGPLNMILD | DGGDLTNLIH | TKYPQLLSGI | 
    200:
    RGISEETTTG | VHNLYKMMSN | GILKVPAINV | NDSVTKSKFD | NLYGCRESLI | 
    250:
    DGIKRATDVM | IAGKVAVVAG | YGDVGKGCAQ | ALRGFGARVI | ITEIDPINAL | 
    300:
    QAAMEGYEVT | TMDEACKEGN | IFVTTTGCVD | IILGRHFEQM | KDDAIVCNIG | 
    350:
    HFDVEIDVKW | LNENAVEKVN | IKPQVDRYWL | KNGRRIILLA | EGRLVNLGCA | 
    400:
    MGHPSFVMSN | SFTNQVMAQI | ELWTHPDKYP | VGVHFLPKKL | DEAVAEAHLG | 
    432:
    KLNVKLTKLT | EKQAQYLGMP | INGPFKPDHY | RY
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    PF05221
    UniGene:
    Mm.330692
    KEGG:
    mmu:269378
    SMR:
    P50247
    String:
    P50247
    Uniprot:
    P50247
     
    FOR
    ELISA Kit for Mouse AdoHcyase
    Cat.:
    E12634h
    Price:
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    Manual:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Mouse AdoHcyase
    Cat.:
    E12634m
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    Manual:
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse AdoHcyase
    MSDS:
    Please sign in first.
    ELISA Kit for Mouse AdoHcyase
    MSDS:
    Please sign in first.
    ELISA Kit for Mouse AdoHcyase
    Cat.:
    E12634r
    Price:
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    Manual:
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Mouse AdoHcyase
    Cat.:
    U12634r
    Price:
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse AdoHcyase
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse AdoHcyase
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse AdoHcyase
    Cat.:
    U12634h
    Price:
    Please sign in first.
    Manual:
    Looking for manual.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse AdoHcyase
    Cat.:
    U12634m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse AdoHcyase
    Cat.:
    P12634Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse AdoHcyase
    Polyclonal Antibody for Mouse AdoHcyase
    Polyclonal Antibody for Mouse AdoHcyase
    Polyclonal Antibody for Mouse AdoHcyase
    Monoclonal Antibody for Mouse AdoHcyase
    Monoclonal Antibody for Mouse AdoHcyase
    Monoclonal Antibody for Mouse AdoHcyase
    Monoclonal Antibody for Mouse AdoHcyase
    Monoclonal Antibody for Mouse AdoHcyase
    Protein for Mouse AdoHcyase
    Protein for Mouse AdoHcyase
    Protein for Mouse AdoHcyase
    Protein for Mouse AdoHcyase
    Protein for Mouse AdoHcyase

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11.7%

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    Recovery
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    Recovery was determined by spiking various levels of SAHH into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    108

    105-113

    Plasma

    106

    102-108

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1：2

    1:4

    1:8

    1:16

    serum(n=5)

    106-112%

    102-112%

    104-110%

    103-114%

    EDTA plasma(n=5)

    103-114%

    104-114%

    100-115%

    105-111%

    heparin plasma(n=5)

    102-103%

     

    103-112%

    101-114%

    106-109%

     

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    References
    1. 1.
      "Identification of a major hepatic copper binding protein as S-adenosylhomocysteine hydrolase."
      Bethin K.E. , Petrovic N. , Ettinger M.J.
      J. Biol. Chem.270:20698-20702(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: BALB/c.
      tissue: Liver.
    2. 2.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Kidney.
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