Ahcy ELISA Kit for Rat(Rattus norvegicus) | EIAab.comAhcy ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Ahcy (Gene name),

Adenosylhomocysteinase (Protein name ), SAHH_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Ahcy;

Protein name:

Adenosylhomocysteinase(AdoHcyase);

Alternative:

S-adenosyl-L-homocysteine hydrolase;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Homotetramer.

Function:

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.

Subcellular Location:

Cytoplasm Melanosome

Protein Attributes:

Sequence length:

432

Sequence:

50:

MADKLPYKVA | DIGLAAWGRK | ALDIAENEMP | GLMRMREMYS | ASKPLKGARI |

100:

AGCLHMTVET | AVLIETLVAL | GAEVRWSSCN | IFSTQDHAAA | AIAKAGIPVF |

150:

AWKGETDEEY | LWCIEQTLHF | KDGPLNMILD | DGGDLTNLIH | TKHPQLLSGI |

200:

RGISEETTTG | VHNLYKMMAN | GILKVPAINV | NDSVTKSKFD | NLYGCRESLI |

250:

DGIKRATDVM | IAGKVAVVAG | YGDVGKGCAQ | ALRGFGARVI | ITEIDPINAL |

300:

QAAMEGYEVT | TMDEACKEGN | IFVTTTGCVD | IILGRHFEQM | KDDAIVCNIG |

350:

HFDVEIDVKW | LNENAVEKVN | IKPQVDRYLL | KNGHRIILLA | EGRLVNLGCA |

400:

MGHPSFVMSN | SFTNQVMAQI | ELWTHPDKYP | VGVHFLPKKL | DEAVAEAHLG |

432:

KLNVKLTKLT | EKQAQYLGMP | INGPFKPDHY | RY

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

KEGG:

UniGene:

STRING:

10116.ENSRNOP00000024310

SMR:

String:

Uniprot:

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ELISA Kit for Rat AdoHcyase

Cat.:

E12634h

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96T

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ELISA Kit for Rat AdoHcyase

Cat.:

E12634m

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96T

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ELISA Kit for Rat AdoHcyase

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ELISA Kit for Rat AdoHcyase

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ELISA Kit for Rat AdoHcyase

Cat.:

E12634r

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96T

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CLIA Kit for Rat AdoHcyase

Cat.:

U12634r

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Packing:

96T

CLIA Kit for Rat AdoHcyase

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CLIA Kit for Rat AdoHcyase

MSDS:

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CLIA Kit for Rat AdoHcyase

Cat.:

U12634h

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Looking for manual.

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96T

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CLIA Kit for Rat AdoHcyase

Cat.:

U12634m

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96T

Polyclonal Antibody for Rat AdoHcyase

Cat.:

P12634Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat AdoHcyase

Polyclonal Antibody for Rat AdoHcyase

Polyclonal Antibody for Rat AdoHcyase

Polyclonal Antibody for Rat AdoHcyase

Monoclonal Antibody for Rat AdoHcyase

Monoclonal Antibody for Rat AdoHcyase

Monoclonal Antibody for Rat AdoHcyase

Monoclonal Antibody for Rat AdoHcyase

Monoclonal Antibody for Rat AdoHcyase

Protein for Rat AdoHcyase

Protein for Rat AdoHcyase

Protein for Rat AdoHcyase

Protein for Rat AdoHcyase

Protein for Rat AdoHcyase

R&D Technical Data

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.7%

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Recovery

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Recovery was determined by spiking various levels of SAHH into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	108	105-113
Plasma	106	102-108

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	106-112%	102-112%	104-110%	103-114%
EDTA plasma(n=5)	103-114%	104-114%	100-115%	105-111%
heparin plasma(n=5)	102-103%	103-112%	101-114%	106-109%

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References

1.

"Amino acid sequence of S-adenosyl-L-homocysteine hydrolase from rat liver as derived from the cDNA sequence."
Ogawa H. , Gomi T. , Mueckler M.M. , Fujioka M. , Backlund P.S. Jr. , Aksamit R.R. , Unson C.G. , Cantoni G.L.
Proc. Natl. Acad. Sci. U.S.A.84:719-723(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
2.

"The gene and pseudogenes of rat S-adenosyl-L-homocysteine hydrolase."
Merta A. , Aksamit R.R. , Kasir J. , Cantoni G.L.
Eur. J. Biochem.229:575-582(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: Fischer 344.
3.

"S-adenosylhomocysteinase from rat liver. Amino acid sequences of the peptides containing active site cysteine residues modified by treatment with 5'-p-fluorosulfonylbenzoyladenosine."
Gomi T. , Ogawa H. , Fujioka M.
J. Biol. Chem.261:13422-13425(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 76-94
4.

Lubec G. , Afjehi-Sadat L. , Diao W.
Submitted (2007-04) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 143-151 AND 268-285;IDENTIFICATION BY MASS SPECTROMETRY
strain: Sprague-Dawley.
tissue: Hippocampus.
tissue: Spinal cord.
5.

"Crystal structure of S-adenosylhomocysteine hydrolase from rat liver."
Hu Y. , Komoto J. , Huang Y. , Gomi T. , Ogawa H. , Takata Y. , Fujioka M. , Takusagawa F.
Biochemistry38:8323-8333(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NAD;COFACTOR;SUBUNIT
tissue: Liver.
6.

"Effects of site-directed mutagenesis on structure and function of recombinant rat liver S-adenosylhomocysteine hydrolase. Crystal structure of D244E mutant enzyme."
Komoto J. , Huang Y. , Gomi T. , Ogawa H. , Takata Y. , Fujioka M. , Takusagawa F.
J. Biol. Chem.275:32147-32156(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF MUTANT GLU-245
7.

"Inhibition of S-adenosylhomocysteine hydrolase by acyclic sugar adenosine analogue D-eritadenine. Crystal structure of S-adenosylhomocysteine hydrolase complexed with D-eritadenine."
Huang Y. , Komoto J. , Takata Y. , Powell D.R. , Gomi T. , Ogawa H. , Fujioka M. , Takusagawa F.
J. Biol. Chem.277:7477-7482(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH INHIBITOR
8.

"Catalytic mechanism of S-adenosylhomocysteine hydrolase. Site-directed mutagenesis of Asp-130, Lys-185, Asp-189, and Asn-190."
Takata Y. , Yamada T. , Huang Y. , Komoto J. , Gomi T. , Ogawa H. , Fujioka M. , Takusagawa F.
J. Biol. Chem.277:22670-22676(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS);MUTAGENESIS OF ASP-131; LYS-186; ASP-190 AND ASN-191

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