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Index > Protein center > STAT5A(Gene name) > Bovine
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  • STAT5A (Gene name),
  • Signal transducer and activator of transcription 5A (Protein name ),  STA5A_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    STAT5A(MGF);
    Protein name:
    Signal transducer and activator of transcription 5A;
    Alternative:
    Mammary gland factor;
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7.
    Function:
    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription.
    Subcellular Location:
    Cytoplasm Nucleus Translocated into the nucleus in response to phosphorylation.
    Protein Attributes:
    Sequence length:
    794
    Sequence:
    50:
    MAGWIQAQQL | QGDALRQMQV | LYGQHFPIEV | RHYLAQWIES | QPWDAIDLDN | 
    100:
    PQDRAQATQL | LEGLVQELQK | KAEHQVGEDG | FLLKIKLGHY | ATQLQNTYDR | 
    150:
    CPMELVRCIR | HILYNEQRLV | REANNGSSSA | GILVDAMSQK | HLQINQTFEE | 
    200:
    LRLVTQDTEN | ELKKLQQTQE | YFIIQYQESL | RIQAQFAQLA | QLNPQERLSR | 
    250:
    ETALQQKQVS | LEAWLQREAQ | TLQQYRVELA | EKHQKTLQLL | RKQQTIILDD | 
    300:
    ELIQWKRRQQ | LAGNGGPPEG | SLDVLQSWCE | KLAEIIWQNR | QQIRRAEHLC | 
    350:
    QQLPIPGPVE | EMLAEVNATI | TDIISALVTS | TFIIEKQPPQ | VLKTQTKFAA | 
    400:
    TVRLLVGGKL | NVHMNPPQVK | ATIISEQQAK | SLLKNENTRN | ECSGEILNNC | 
    450:
    CVMEYHQATG | TLSAHFRNMS | LKRIKRADRR | GAESVTEEKF | TVLFESQFSV | 
    500:
    GSNELVFQVK | TLSLPVVVIV | HGSQDHNATA | TVLWDNAFAE | PGRVPFAVPD | 
    550:
    KVLWPQLCEA | LNMKFKAEVQ | SNRGLTKENL | VFLAQKLFNS | SSSHLEDYNG | 
    600:
    MSVSWSQFNR | ENLPGWNYTF | WQWFDGVMEV | LKKHHKPHWN | DGAILGFVNK | 
    650:
    QQAHDLLINK | PDGTFLLRFS | DSEIGGITIA | WKFDSPDRNL | WNLKPFTTRD | 
    700:
    FSIRSLADRL | GDLNYLIYVF | PDRPKDEVFS | KYYTPVLAKA | VDGYVKPQIK | 
    750:
    QVVPEFVSAS | ADSAGSNATY | MDQAPSPAVC | PQPHYNMYPQ | NPDPVLDQDG | 
    794:
    EFDLDETMDV | ARHVEELLRR | PMDSLEPSLP | PPTGLFTPGR | GSLS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    Pfam:
    SMR:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    E1738m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    E1738b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    E1738h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    E1738r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    E1738p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    U1738m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    U1738p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    U1738r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    U1738h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    U1738b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Polyclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
    Protein for Bovine Signal transducer and activator of transcription 5A
    Protein for Bovine Signal transducer and activator of transcription 5A
    Protein for Bovine Signal transducer and activator of transcription 5A
    Protein for Bovine Signal transducer and activator of transcription 5A
    Protein for Bovine Signal transducer and activator of transcription 5A

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤12.4%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery
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    Recovery was determined by spiking various levels of STA5A into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STA5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    91-105%

    92-103%

    95-100%

    93-102%

    EDTA plasma(n=5)

    91-105%

    94-102%

    93-101%

    95-101%

    heparin plasma(n=5)

    90-104%

     

    95-100%

    95-99%

    92-104%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Alternative STAT5A/MGF-factors in the mammary gland of ruminants."
      Schroeder P. , Thiesen H.-J. , Seyfert H.-M.
      Submitted (1996-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Mammary gland.
    2. 2.
      "Molecular characterization of STAT5A- and STAT5B-encoding genes reveals extended intragenic sequence homogeneity in cattle and mouse and different degrees of divergent evolution of various domains."
      Seyfert H.-M. , Pitra C. , Meyer L. , Brunner R.M. , Wheeler T.T. , Molenaar A. , McCracken J.Y. , Herrmann J. , Thiesen H.-J. , Schwerin M.
      J. Mol. Evol.50:550-561(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Cloning and sequencing of the bovine STAT5A cDNA reveals significant sequence divergence with ovine."
      Schroeder P. , Meyer L. , Wheeler T.T. , Thiesen H.-J. , Seyfert H.-M.
      Submitted (1998-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95
      tissue: Blood.
    4. 4.
      "A polymorphic TG repeat present within the bovine STAT5A gene."
      McCracken J.Y. , Molenaar A.J. , Snell R.J. , Dayey H.W. , Wilkins R.J.
      Anim. Genet.28:459-459(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 461-546
    5. 5.
      "A single strand conformational polymorphism in the bovine gene STAT5A."
      Antoniou E. , Hirst B.J. , Grosz M. , Skidmore C.J.
      Anim. Genet.30:232-232(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 582-677
    6. 6.
      "Bos taurus partial sequence of STAT5a gene."
      LeBaron M.J. , Yamashita H. , Rui H.
      Submitted (2000-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 748-794
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