Signal transducer and activator of transcription 5A (Protein name
), STA5A_BOVIN from NCBI database.
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General Annotation
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Antigen Annotation
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Gene name:
STAT5A(MGF);
Protein name:
Signal transducer and activator of transcription 5A;
Alternative:
Mammary gland factor;
Organism:
Bovine (Bos taurus).
General Annotation
Sub Unit:
Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7.
Function:
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription.
Subcellular Location:
Cytoplasm
Nucleus
Translocated into the nucleus in response to phosphorylation.
Polyclonal Antibody for Bovine Signal transducer and activator of transcription 5A
Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
Monoclonal Antibody for Bovine Signal transducer and activator of transcription 5A
Protein for Bovine Signal transducer and activator of transcription 5A
Protein for Bovine Signal transducer and activator of transcription 5A
Protein for Bovine Signal transducer and activator of transcription 5A
Protein for Bovine Signal transducer and activator of transcription 5A
Protein for Bovine Signal transducer and activator of transcription 5A
R&D Technical Data
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s
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤12.4%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
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Recovery was determined by spiking various levels of STA5A into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
91-103
Plasma
98
93-106
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STA5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
91-105%
92-103%
95-100%
93-102%
EDTA plasma(n=5)
91-105%
94-102%
93-101%
95-101%
heparin plasma(n=5)
90-104%
95-100%
95-99%
92-104%
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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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References
1.
"Alternative STAT5A/MGF-factors in the mammary gland of ruminants." Schroeder P.
,
Thiesen H.-J.
,
Seyfert H.-M.
Submitted (1996-08) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]