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Index > Protein center > STAT5A(Gene name) > Human
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  • STAT5A (Gene name),
  • Signal transducer and activator of transcription 5A (Protein name ),  STA5A_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    STAT5A(STAT5);
    Protein name:
    Signal transducer and activator of transcription 5A;
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7.
    Function:
    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription.
    Subcellular Location:
    Cytoplasm Nucleus Translocated into the nucleus in response to phosphorylation.
    Protein Attributes:
    Sequence length:
    794
    Sequence:
    50:
    MAGWIQAQQL | QGDALRQMQV | LYGQHFPIEV | RHYLAQWIES | QPWDAIDLDN | 
    100:
    PQDRAQATQL | LEGLVQELQK | KAEHQVGEDG | FLLKIKLGHY | ATQLQKTYDR | 
    150:
    CPLELVRCIR | HILYNEQRLV | REANNCSSPA | GILVDAMSQK | HLQINQTFEE | 
    200:
    LRLVTQDTEN | ELKKLQQTQE | YFIIQYQESL | RIQAQFAQLA | QLSPQERLSR | 
    250:
    ETALQQKQVS | LEAWLQREAQ | TLQQYRVELA | EKHQKTLQLL | RKQQTIILDD | 
    300:
    ELIQWKRRQQ | LAGNGGPPEG | SLDVLQSWCE | KLAEIIWQNR | QQIRRAEHLC | 
    350:
    QQLPIPGPVE | EMLAEVNATI | TDIISALVTS | TFIIEKQPPQ | VLKTQTKFAA | 
    400:
    TVRLLVGGKL | NVHMNPPQVK | ATIISEQQAK | SLLKNENTRN | ECSGEILNNC | 
    450:
    CVMEYHQATG | TLSAHFRNMS | LKRIKRADRR | GAESVTEEKF | TVLFESQFSV | 
    500:
    GSNELVFQVK | TLSLPVVVIV | HGSQDHNATA | TVLWDNAFAE | PGRVPFAVPD | 
    550:
    KVLWPQLCEA | LNMKFKAEVQ | SNRGLTKENL | VFLAQKLFNN | SSSHLEDYSG | 
    600:
    LSVSWSQFNR | ENLPGWNYTF | WQWFDGVMEV | LKKHHKPHWN | DGAILGFVNK | 
    650:
    QQAHDLLINK | PDGTFLLRFS | DSEIGGITIA | WKFDSPERNL | WNLKPFTTRD | 
    700:
    FSIRSLADRL | GDLSYLIYVF | PDRPKDEVFS | KYYTPVLAKA | VDGYVKPQIK | 
    750:
    QVVPEFVNAS | ADAGGSSATY | MDQAPSPAVC | PQAPYNMYPQ | NPDHVLDQDG | 
    794:
    EFDLDETMDV | ARHVEELLRR | PMDSLDSRLS | PPAGLFTSAR | GSLS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    Pfam:
    MIM:
    KEGG:
    UniGene:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    E1738m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    E1738b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    E1738h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    E1738r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    E1738p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    U1738m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    U1738p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    U1738r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    U1738h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Signal transducer and activator of transcription 5A
    Cat.:
    U1738b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Signal transducer and activator of transcription 5A
    Polyclonal Antibody for Human Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Human Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Human Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Human Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Human Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Human Signal transducer and activator of transcription 5A
    Protein for Human Signal transducer and activator of transcription 5A
    Protein for Human Signal transducer and activator of transcription 5A
    Protein for Human Signal transducer and activator of transcription 5A
    Protein for Human Signal transducer and activator of transcription 5A
    Protein for Human Signal transducer and activator of transcription 5A

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤12.4%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery
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    Recovery was determined by spiking various levels of STA5A into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STA5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    91-105%

    92-103%

    95-100%

    93-102%

    EDTA plasma(n=5)

    91-105%

    94-102%

    93-101%

    95-101%

    heparin plasma(n=5)

    90-104%

     

    95-100%

    95-99%

    92-104%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Identification and purification of human Stat proteins activated in response to interleukin-2."
      Hou J. , Schindler U. , Henzel W.J. , Wong S.C. , McKnight S.L.
      Immunity2:321-329(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    2. 2.
      Lin J.X. , Mietz J. , Modi W.S. , John S. , Leonard W.J.
      Submitted (1995-12) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    3. 3.
      "Cloning and characterization of a variant of human stat5a, missing a portion of the n-terminal region, with dominant negative effects on the growth of breast cancer cells and [beta]-casein gene expression."
      Tan D. , Deng C. , Luben R.A. , Walker A.M.
      Submitted (2006-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);ALTERNATIVE SPLICING
    4. 4.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
      tissue: Synovium.
    5. 6.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Cervix.
    6. 7.
      "A nuclear protein tyrosine phosphatase TC-PTP is a potential negative regulator of the PRL-mediated signaling pathway: dephosphorylation and deactivation of signal transducer and activator of transcription 5a and 5b by TC-PTP in nucleus."
      Aoki N. , Matsuda T.
      Mol. Endocrinol.16:58-69(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PROLACTIN SIGNALING PATHWAY;PHOSPHORYLATION;DEPHOSPHORYLATION BY PTPN2
    7. 8.
      "NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain."
      Litterst C.M. , Kliem S. , Marilley D. , Pfitzner E.
      J. Biol. Chem.278:45340-45351(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH NCOA1
    8. 9.
      "STAT5a activation mediates the epithelial to mesenchymal transition induced by oncogenic RhoA."
      Benitah S.A. , Valeron P.F. , Rui H. , Lacal J.C.
      Mol. Biol. Cell14:40-53(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-694 BY JAK2
    9. 10.
      "FLT3 mutations in the activation loop of tyrosine kinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy."
      Taketani T. , Taki T. , Sugita K. , Furuichi Y. , Ishii E. , Hanada R. , Tsuchida M. , Sugita K. , Ida K. , Hayashi Y.
      Blood103:1085-1088(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION IN RESPONSE TO FLT3 SIGNALING
    10. 11.
      "Signal transduction via the stem cell factor receptor/c-Kit."
      Ronnstrand L.
      Cell. Mol. Life Sci.61:2535-2548(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON ROLE IN KIT SIGNALING
    11. 12.
      "The ERBB4/HER4 receptor tyrosine kinase regulates gene expression by functioning as a STAT5A nuclear chaperone."
      Williams C.C. , Allison J.G. , Vidal G.A. , Burow M.E. , Beckman B.S. , Marrero L. , Jones F.E.
      J. Cell Biol.167:469-478(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ERBB4;SUBCELLULAR LOCATION;FUNCTION
    12. 13.
      "Suppressor of cytokine signaling 7 inhibits prolactin, growth hormone, and leptin signaling by interacting with STAT5 or STAT3 and attenuating their nuclear translocation."
      Martens N. , Uzan G. , Wery M. , Hooghe R. , Hooghe-Peters E.L. , Gertler A.
      J. Biol. Chem.280:13817-13823(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SOCS7
    13. 14.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    14. 15.
      "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
      Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    15. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    16. 17.
      "Mechanisms of STAT protein activation by oncogenic KIT mutants in neoplastic mast cells."
      Chaix A. , Lopez S. , Voisset E. , Gros L. , Dubreuil P. , De Sepulveda P.
      J. Biol. Chem.286:5956-5966(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-694 IN RESPONSE TO KIT SIGNALING
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