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Index > Protein center > Stat5a(Gene name) > Rat
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  • Stat5a (Gene name),
  • Signal transducer and activator of transcription 5A (Protein name ),  STA5A_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Stat5a(Mgf);
    Protein name:
    Signal transducer and activator of transcription 5A;
    Alternative:
    Mammary gland factor;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7.
    Function:
    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription.
    Subcellular Location:
    Cytoplasm Nucleus Mostly cytoplasmic during pregnancy, whereas it becomes predominantly nuclear during early lactation. It is translocated into the nucleus in response to phosphorylation.
    Protein Attributes:
    Sequence length:
    793
    Sequence:
    50:
    MAGWIQAQQL | QGDALRQMQV | LYGQHFPIEV | RHYLAQWIES | QPWDAIDLDN | 
    100:
    PQDRGQATQL | LEGLVQELQK | KAEHQVGEDG | FVLKIKLGHY | ATQLQNTYDR | 
    150:
    CPMELVRCIR | HILYNEQRLV | REANNCSSPA | GVLVDAMSQK | HLQINQTFEE | 
    200:
    LRLITQDTES | ELKKLQQTQE | YFIIQYQESL | RIQAQFGQLA | QLNPQERMSR | 
    250:
    ETALQQKQVS | LETWLQREAQ | TLQQYRVELV | EKHQKTLQLL | RKQQTIILDD | 
    300:
    ELIQWKRGQQ | LAGNGGPPEG | SLDVLQSWCE | KLAEIIWQNR | QQIRRAEHLC | 
    350:
    QQLPIPGPVE | EMLAEVNATI | TDIISALVTS | TFIIEKQPPQ | VLKTQTKFAA | 
    400:
    TVRLLVGGKL | NVHMNPPQVK | ATIISEQQAK | SLLKNENTRN | DYSGEILNNC | 
    450:
    CVMEYHQATG | TLSAHFRNMS | LKRIKRADRR | GAESVTEEKF | TVLFESQFSV | 
    500:
    GSNELVFQVK | TLSLPVVVIV | HGSQDHNATA | TVLWDNAFAE | PGRVPFAVPD | 
    550:
    KVLWPQLCEA | LNMKFKAEVQ | SNRGLTKENL | VFLAQKLFNR | RSNHLEDYNS | 
    600:
    MSVSWSQFNR | ENLPGWNYTF | WQWFDGVMEV | LKKHHKPHWN | DGAILGFVNK | 
    650:
    QQAHDLLINK | PDGTFLLRFS | DSEIGGITIA | WKFDSPDRNL | WNLKPFTTRD | 
    700:
    FSIRSLADRL | GDLNYLIYVF | PDRPKDEVFA | KYYTPVLAKA | VDGYVKPQIK | 
    750:
    QVVPEFVNAS | ADAGANATYM | DQAPSPVVCP | QAHYNMYPPN | PDPVLDQDGE | 
    793:
    FDLDETMDVA | RHVEELLRRP | MDSLDPRLSP | PAGLFTSARS | SLS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    UniGene:
    Pfam:
    String:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    E1738m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    E1738b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    E1738h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    E1738r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    E1738p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    U1738m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    U1738p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    U1738r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    U1738h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Signal transducer and activator of transcription 5A
    Cat.:
    U1738b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Polyclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Cat.:
    P1738Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Monoclonal Antibody for Rat Signal transducer and activator of transcription 5A
    Protein for Rat Signal transducer and activator of transcription 5A
    Protein for Rat Signal transducer and activator of transcription 5A
    Protein for Rat Signal transducer and activator of transcription 5A
    Protein for Rat Signal transducer and activator of transcription 5A
    Protein for Rat Signal transducer and activator of transcription 5A

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤12.4%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery
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    Recovery was determined by spiking various levels of STA5A into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STA5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    91-105%

    92-103%

    95-100%

    93-102%

    EDTA plasma(n=5)

    91-105%

    94-102%

    93-101%

    95-101%

    heparin plasma(n=5)

    90-104%

     

    95-100%

    95-99%

    92-104%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Regulation of mammary gland factor/Stat5a during mammary gland development."
      Kazansky A.V. , Raught B. , Lindsey S.M. , Wang Y.-F. , Rosen J.M.
      Mol. Endocrinol.9:1598-1609(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STAT5A1 AND STAT5A2)
      tissue: Mammary gland.
    2. 2.
      "Analysis of STAT1 activation by six FGFR3 mutants associated with skeletal dysplasia undermines dominant role of STAT1 in FGFR3 signaling in cartilage."
      Krejci P. , Salazar L. , Kashiwada T.A. , Chlebova K. , Salasova A. , Thompson L.M. , Bryja V. , Kozubik A. , Wilcox W.R.
      PLoS ONE3:E3961-E3961(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION AT TYR-694 IN RESPONSE TO CONSTITUTIVELY ACTIVATED FGFR3
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