Metalloproteinase inhibitor 4 (Protein name
), TIMP4_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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Gene name:
TIMP4;
Protein name:
Metalloproteinase inhibitor 4;
Alternative:
Tissue inhibitor of metalloproteinases 4(TIMP-4);
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
N/A
Function:
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Monoclonal Antibody for Human Metalloproteinase inhibitor 4
Monoclonal Antibody for Human Metalloproteinase inhibitor 4
Monoclonal Antibody for Human Metalloproteinase inhibitor 4
Monoclonal Antibody for Human Metalloproteinase inhibitor 4
Monoclonal Antibody for Human Metalloproteinase inhibitor 4
Protein for Human Metalloproteinase inhibitor 4
Protein for Human Metalloproteinase inhibitor 4
Protein for Human Metalloproteinase inhibitor 4
Protein for Human Metalloproteinase inhibitor 4
Protein for Human Metalloproteinase inhibitor 4
R&D Technical Data
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤8.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤12.6%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
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Recovery was determined by spiking various levels of TIMP4 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
96
92-101
Plasma
97
95-99
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-99%
93-98%
90-102%
93-100%
Saliva(n=3)
91-102%
91-100%
92-100%
90-104%
heparin plasma(n=5)
93-99%
90-103%
94-99%
92-102%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
Saliva(n=3)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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