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Index > Protein center > TIMP4(Gene name) > Human
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  • TIMP4 (Gene name),
  • Metalloproteinase inhibitor 4 (Protein name ),  TIMP4_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    TIMP4;
    Protein name:
    Metalloproteinase inhibitor 4;
    Alternative:
    Tissue inhibitor of metalloproteinases 4(TIMP-4);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    224
    Sequence:
    50:
    MPGSPRPAPS | WVLLLRLLAL | LRPPGLGEAC | SCAPAHPQQH | ICHSALVIRA | 
    100:
    KISSEKVVPA | SADPADTEKM | LRYEIKQIKM | FKGFEKVKDV | QYIYTPFDSS | 
    150:
    LCGVKLEANS | QKQYLLTGQV | LSDGKVFIHL | CNYIEPWEDL | SLVQRESLNH | 
    200:
    HYHLNCGCQI | TTCYTVPCTI | SAPNECLWTD | WLLERKLYGY | QAQHYVCMKH | 
    224:
    VDGTCSWYRG | HLPLRKEFVD | IVQP
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    MIM:
    KEGG:
    Pfam:
    UniGene:
    SMR:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    E0130r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    E0130m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Metalloproteinase inhibitor 4
    MSDS:
    Please sign in first.
    ELISA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    E0130b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    E0130h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    U0130h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    U0130b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Metalloproteinase inhibitor 4
    MSDS:
    Please sign in first.
    CLIA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    U0130r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Metalloproteinase inhibitor 4
    Cat.:
    U0130m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Metalloproteinase inhibitor 4
    Polyclonal Antibody for Human Metalloproteinase inhibitor 4
    Cat.:
    P0130Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Metalloproteinase inhibitor 4
    Cat.:
    P0130Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Metalloproteinase inhibitor 4
    Polyclonal Antibody for Human Metalloproteinase inhibitor 4
    Cat.:
    P0130Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Metalloproteinase inhibitor 4
    Monoclonal Antibody for Human Metalloproteinase inhibitor 4
    Monoclonal Antibody for Human Metalloproteinase inhibitor 4
    Monoclonal Antibody for Human Metalloproteinase inhibitor 4
    Monoclonal Antibody for Human Metalloproteinase inhibitor 4
    Protein for Human Metalloproteinase inhibitor 4
    Protein for Human Metalloproteinase inhibitor 4
    Protein for Human Metalloproteinase inhibitor 4
    Protein for Human Metalloproteinase inhibitor 4
    Protein for Human Metalloproteinase inhibitor 4

    R&D Technical Data
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    s

    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.4%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤12.6%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of TIMP4 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    96

    92-101

    Plasma

    97

    95-99

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-99%

    93-98%

    90-102%

    93-100%

    Saliva(n=3)

    91-102%

    91-100%

    92-100%

    90-104%

    heparin plasma(n=5)

    93-99%

     

    90-103%

    94-99%

    92-102%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4."
      Greene J. , Wang M. , Liu Y.E. , Raymond L.A. , Rosen C. , Shi Y.E.
      J. Biol. Chem.271:30375-30380(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Heart.
    2. 2.
      "Cloning of the human tissue inhibitor of metalloproteinase-4 gene (TIMP4) and localization of the TIMP4 and timp4 genes to human chromosome 3p25 and mouse chromosome 6, respectively."
      Olson T.M. , Hirohata S. , Ye J. , Leco K. , Seldin M.F. , Apte S.S.
      Genomics51:148-151(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    4. 4.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Cerebellum.
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Brain.
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