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Index > Protein center > AZGP1(Gene name) > Human
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  • AZGP1 (Gene name),
  • Zinc-alpha-2-glycoprotein (Protein name ),  ZA2G_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    AZGP1(ZAG;ZNGP1);
    Protein name:
    Zinc-alpha-2-glycoprotein(Zn-alpha-2-GP;Zn-alpha-2-glycoprotein);
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with PIP.
    Function:
    Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    298
    Sequence:
    50:
    MVRMVPVLLS | LLLLLGPAVP | QENQDGRYSL | TYIYTGLSKH | VEDVPAFQAL | 
    100:
    GSLNDLQFFR | YNSKDRKSQP | MGLWRQVEGM | EDWKQDSQLQ | KAREDIFMET | 
    150:
    LKDIVEYYND | SNGSHVLQGR | FGCEIENNRS | SGAFWKYYYD | GKDYIEFNKE | 
    200:
    IPAWVPFDPA | AQITKQKWEA | EPVYVQRAKA | YLEEECPATL | RKYLKYSKNI | 
    250:
    LDRQDPPSVV | VTSHQAPGEK | KKLKCLAYDF | YPGKIDVHWT | RAGEVQEPEL | 
    298:
    RGDVLHNGNG | TYQSWVVVAV | PPQDTAPYSC | HVQHSSLAQP | LVVPWEAS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    KEGG:
    Pfam:
    UniGene:
    SMR:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Zn-alpha-2-GP
    Cat.:
    E2245h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Zn-alpha-2-GP
    Cat.:
    E2245m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Zn-alpha-2-GP
    Cat.:
    E2245b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Zn-alpha-2-GP
    Cat.:
    E2245r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Zn-alpha-2-GP
    Cat.:
    U2245m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Zn-alpha-2-GP
    Cat.:
    U2245h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Zn-alpha-2-GP
    Cat.:
    U2245r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Zn-alpha-2-GP
    Cat.:
    U2245b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Zn-alpha-2-GP
    Cat.:
    P2245Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Zn-alpha-2-GP
    Cat.:
    P2245Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Zn-alpha-2-GP
    Cat.:
    P2245Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Zn-alpha-2-GP
    Monoclonal Antibody for Human Zn-alpha-2-GP
    Monoclonal Antibody for Human Zn-alpha-2-GP
    Monoclonal Antibody for Human Zn-alpha-2-GP
    Monoclonal Antibody for Human Zn-alpha-2-GP
    Protein for Human Zn-alpha-2-GP
    Protein for Human Zn-alpha-2-GP
    Protein for Human Zn-alpha-2-GP
    Protein for Human Zn-alpha-2-GP

    R&D Technical Data
    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.4%

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    Recovery

    Recovery was determined by spiking various levels of AZGP1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    97-103

    Plasma

    102

    95-104

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AZGP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    100-105%

    94-96%

    102-108%

    96-104%

    EDTA plasma(n=5)

    97-106%

    95-104%

    93-102%

    100-105%

    heparin plasma(n=5)

    102-107%

     

    100-105%

    98-106%

    97-104%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Human Zn-alpha 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues."
      Freije J.P. , Fueyo A. , Uria J. , Lopez-Otin C.
      FEBS Lett.290:247-249(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Mammary gland.
    2. 2.
      "Molecular cloning and chromosomal assignment of the gene for human Zn-alpha 2-glycoprotein."
      Ueyama H. , Deng H.X. , Ohkubo I.
      Biochemistry32:12968-12976(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Human Zn-alpha 2-glycoprotein: complete genomic sequence, identification of a related pseudogene and relationship to class I major histocompatibility complex genes."
      Freije J.P. , Fueyo A. , Uria J.A. , Velasco G. , Sanchez L.M. , Lopez-Boado Y.S. , Lopez-Otin C.
      Genomics18:575-587(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      tissue: Leukocyte.
    4. 4.
      "The DNA sequence of human chromosome 7."
      Hillier L.W. , Fulton R.S. , Fulton L.A. , Graves T.A. , Pepin K.H. , Wagner-McPherson C. , Layman D. , Maas J. , Jaeger S. , Walker R. , Wylie K. , Sekhon M. , Becker M.C. , O'Laughlin M.D. , Schaller M.E. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , more...
      Nature424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    5. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Colon.
      tissue: Prostate.
    7. 8.
      "Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene."
      Ueyama H. , Niwa M. , Tada T. , Sasaki M. , Ohkubo I.
      Biochem. Biophys. Res. Commun.177:696-703(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-298
      tissue: Liver.
      tissue: Prostate.
    8. 9.
      "Complete amino acid sequence of human plasma Zn-alpha 2-glycoprotein and its homology to histocompatibility antigens."
      Araki T. , Gejyo F. , Takagaki K. , Haupt H. , Schwick H.G. , Buergi W. , Marti T. , Schaller J. , Rickli E. , Brossmer R. , Atkinson P.H. , Putnam F.W. , Schmid K.
      Proc. Natl. Acad. Sci. U.S.A.85:679-683(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-298;PYROGLUTAMATE FORMATION AT GLN-21
      tissue: Plasma.
    9. 10.
      "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z. , Henzel W.J.
      Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-35
    10. 11.
      "Biochemical characterization and crystallization of human Zn-alpha2-glycoprotein, a soluble class I major histocompatibility complex homolog."
      Sanchez L.M. , Lopez-Otin C. , Bjorkman P.J.
      Proc. Natl. Acad. Sci. U.S.A.94:4626-4630(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION;CRYSTALLIZATION
    11. 12.
      "Hydrophobic ligand binding by Zn-alpha 2-glycoprotein, a soluble fat-depleting factor related to major histocompatibility complex proteins."
      Kennedy M.W. , Heikema A.P. , Cooper A. , Bjorkman P.J. , Sanchez L.M.
      J. Biol. Chem.276:35008-35013(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IN VITRO BINDING OF FATTY ACID
    12. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-128
      tissue: Bile.
    13. 14.
      "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."
      Bunkenborg J. , Pilch B.J. , Podtelejnikov A.V. , Wisniewski J.R.
      Proteomics4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-112 AND ASN-128
      tissue: Plasma.
    14. 15.
      "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
      J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-112
      tissue: Plasma.
    15. 16.
      "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
      Ramachandran P. , Boontheung P. , Xie Y. , Sondej M. , Wong D.T. , Loo J.A.
      J. Proteome Res.5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109; ASN-112 AND ASN-128
      tissue: Saliva.
    16. 17.
      "Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
      Picariello G. , Ferranti P. , Mamone G. , Roepstorff P. , Addeo F.
      Proteomics8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109; ASN-112 AND ASN-128
      tissue: Milk.
    17. 18.
      "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
      J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109; ASN-112 AND ASN-128
      tissue: Liver.
    18. 19.
      "A strategy for precise and large scale identification of core fucosylated glycoproteins."
      Jia W. , Lu Z. , Fu Y. , Wang H.P. , Wang L.H. , Chi H. , Yuan Z.F. , Zheng Z.B. , Song L.N. , Han H.H. , Liang Y.M. , Wang J.L. , Cai Y. , Zhang Y.K. , Deng Y.L. , Ying W.T. , He S.M. , Qian X.H.
      Mol. Cell. Proteomics8:913-923(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-109 AND ASN-128
    19. 20.
      "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
      Halim A. , Nilsson J. , Ruetschi U. , Hesse C. , Larson G.
      Mol. Cell. Proteomics0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-128;STRUCTURE OF CARBOHYDRATES;IDENTIFICATION BY MASS SPECTROMETRY
    20. 21.
      "Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules."
      Sanchez L.M. , Chirino A.J. , Bjorkman P.J.
      Science283:1914-1919(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS);DISULFIDE BONDS;GLYCOSYLATION AT ASN-109; ASN-128 AND ASN-259
    21. 22.
      "Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein."
      Delker S.L. , West A.P. Jr. , McDermott L. , Kennedy M.W. , Bjorkman P.J.
      J. Struct. Biol.148:205-213(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 21-298;DISULFIDE BONDS;GLYCOSYLATION AT ASN-128 AND ASN-259
    22. 23.
      "Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma."
      Hassan M.I. , Bilgrami S. , Kumar V. , Singh N. , Yadav S. , Kaur P. , Singh T.P.
      J. Mol. Biol.384:663-672(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 32-149 IN COMPLEX WITH PIP;DISULFIDE BONDS;GLYCOSYLATION AT ASN-259
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