AZGP1 ELISA Kit for Human(Homo sapiens) | EIAab.comAZGP1 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > AZGP1(Gene name) > Human

AZGP1 (Gene name),

Zinc-alpha-2-glycoprotein (Protein name ), ZA2G_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

AZGP1(ZAG;ZNGP1);

Protein name:

Zinc-alpha-2-glycoprotein(Zn-alpha-2-GP;Zn-alpha-2-glycoprotein);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with PIP.

Function:

Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

298

Sequence:

50:

100:

150:

200:

250:

298:

RGDVLHNGNG | TYQSWVVVAV | PPQDTAPYSC | HVQHSSLAQP | LVVPWEAS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P25311

KEGG:

hsa:563

Pfam:

PF07654

UniGene:

Hs.546239

SMR:

P25311

STRING:

9606.ENSP00000292401

MIM:

194460

Uniprot:

P25311

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ELISA Kit for Human Zn-alpha-2-GP

Cat.:

E2245h

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Packing:

96T

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ELISA Kit for Human Zn-alpha-2-GP

Cat.:

E2245m

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Looking for manual.

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96T

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ELISA Kit for Human Zn-alpha-2-GP

Cat.:

E2245b

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96T

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ELISA Kit for Human Zn-alpha-2-GP

Cat.:

E2245r

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Packing:

96T

CLIA Kit for Human Zn-alpha-2-GP

Cat.:

U2245m

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96T

CLIA Kit for Human Zn-alpha-2-GP

Cat.:

U2245h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human Zn-alpha-2-GP

Cat.:

U2245r

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MSDS:

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Packing:

96T

CLIA Kit for Human Zn-alpha-2-GP

Cat.:

U2245b

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96T

Polyclonal Antibody for Human Zn-alpha-2-GP

Cat.:

P2245Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Zn-alpha-2-GP

Cat.:

P2245Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Zn-alpha-2-GP

Cat.:

P2245Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Zn-alpha-2-GP

Monoclonal Antibody for Human Zn-alpha-2-GP

Protein for Human Zn-alpha-2-GP

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of AZGP1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	97-103
Plasma	102	95-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AZGP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	94-96%	102-108%	96-104%
EDTA plasma(n=5)	97-106%	95-104%	93-102%	100-105%
heparin plasma(n=5)	102-107%	100-105%	98-106%	97-104%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Human Zn-alpha 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues."
Freije J.P. , Fueyo A. , Uria J. , Lopez-Otin C.
FEBS Lett.290:247-249(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Mammary gland.
2.

"Molecular cloning and chromosomal assignment of the gene for human Zn-alpha 2-glycoprotein."
Ueyama H. , Deng H.X. , Ohkubo I.
Biochemistry32:12968-12976(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Human Zn-alpha 2-glycoprotein: complete genomic sequence, identification of a related pseudogene and relationship to class I major histocompatibility complex genes."
Freije J.P. , Fueyo A. , Uria J.A. , Velasco G. , Sanchez L.M. , Lopez-Boado Y.S. , Lopez-Otin C.
Genomics18:575-587(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Leukocyte.
4.

"The DNA sequence of human chromosome 7."
Hillier L.W. , Fulton R.S. , Fulton L.A. , Graves T.A. , Pepin K.H. , Wagner-McPherson C. , Layman D. , Maas J. , Jaeger S. , Walker R. , Wylie K. , Sekhon M. , Becker M.C. , O'Laughlin M.D. , Schaller M.E. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , more...
Nature424:157-164(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"Human chromosome 7: DNA sequence and biology."
Scherer S.W. , Cheung J. , MacDonald J.R. , Osborne L.R. , Nakabayashi K. , Herbrick J.-A. , Carson A.R. , Parker-Katiraee L. , Skaug J. , Khaja R. , Zhang J. , Hudek A.K. , Li M. , Haddad M. , Duggan G.E. , Fernandez B.A. , Kanematsu E. , Gentles S. , more...
Science300:767-772(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Colon.
tissue: Prostate.
8.

"Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene."
Ueyama H. , Niwa M. , Tada T. , Sasaki M. , Ohkubo I.
Biochem. Biophys. Res. Commun.177:696-703(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-298
tissue: Liver.
tissue: Prostate.
9.

"Complete amino acid sequence of human plasma Zn-alpha 2-glycoprotein and its homology to histocompatibility antigens."
Araki T. , Gejyo F. , Takagaki K. , Haupt H. , Schwick H.G. , Buergi W. , Marti T. , Schaller J. , Rickli E. , Brossmer R. , Atkinson P.H. , Putnam F.W. , Schmid K.
Proc. Natl. Acad. Sci. U.S.A.85:679-683(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-298;PYROGLUTAMATE FORMATION AT GLN-21
tissue: Plasma.
10.

"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z. , Henzel W.J.
Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-35
11.

"Biochemical characterization and crystallization of human Zn-alpha2-glycoprotein, a soluble class I major histocompatibility complex homolog."
Sanchez L.M. , Lopez-Otin C. , Bjorkman P.J.
Proc. Natl. Acad. Sci. U.S.A.94:4626-4630(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION;CRYSTALLIZATION
12.

"Hydrophobic ligand binding by Zn-alpha 2-glycoprotein, a soluble fat-depleting factor related to major histocompatibility complex proteins."
Kennedy M.W. , Heikema A.P. , Cooper A. , Bjorkman P.J. , Sanchez L.M.
J. Biol. Chem.276:35008-35013(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IN VITRO BINDING OF FATTY ACID
13.

"A proteomic analysis of human bile."
Kristiansen T.Z. , Bunkenborg J. , Gronborg M. , Molina H. , Thuluvath P.J. , Argani P. , Goggins M.G. , Maitra A. , Pandey A.
Mol. Cell. Proteomics3:715-728(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-128
tissue: Bile.
14.

"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."
Bunkenborg J. , Pilch B.J. , Podtelejnikov A.V. , Wisniewski J.R.
Proteomics4:454-465(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-112 AND ASN-128
tissue: Plasma.
15.

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-112
tissue: Plasma.
16.

"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
Ramachandran P. , Boontheung P. , Xie Y. , Sondej M. , Wong D.T. , Loo J.A.
J. Proteome Res.5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109; ASN-112 AND ASN-128
tissue: Saliva.
17.

"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
Picariello G. , Ferranti P. , Mamone G. , Roepstorff P. , Addeo F.
Proteomics8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109; ASN-112 AND ASN-128
tissue: Milk.
18.

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109; ASN-112 AND ASN-128
tissue: Liver.
19.

"A strategy for precise and large scale identification of core fucosylated glycoproteins."
Jia W. , Lu Z. , Fu Y. , Wang H.P. , Wang L.H. , Chi H. , Yuan Z.F. , Zheng Z.B. , Song L.N. , Han H.H. , Liang Y.M. , Wang J.L. , Cai Y. , Zhang Y.K. , Deng Y.L. , Ying W.T. , He S.M. , Qian X.H.
Mol. Cell. Proteomics8:913-923(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-109 AND ASN-128
20.

"Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
Halim A. , Nilsson J. , Ruetschi U. , Hesse C. , Larson G.
Mol. Cell. Proteomics0:0-0(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-128;STRUCTURE OF CARBOHYDRATES;IDENTIFICATION BY MASS SPECTROMETRY
21.

"Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules."
Sanchez L.M. , Chirino A.J. , Bjorkman P.J.
Science283:1914-1919(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS);DISULFIDE BONDS;GLYCOSYLATION AT ASN-109; ASN-128 AND ASN-259
22.

"Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein."
Delker S.L. , West A.P. Jr. , McDermott L. , Kennedy M.W. , Bjorkman P.J.
J. Struct. Biol.148:205-213(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 21-298;DISULFIDE BONDS;GLYCOSYLATION AT ASN-128 AND ASN-259
23.

"Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma."
Hassan M.I. , Bilgrami S. , Kumar V. , Singh N. , Yadav S. , Kaur P. , Singh T.P.
J. Mol. Biol.384:663-672(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 32-149 IN COMPLEX WITH PIP;DISULFIDE BONDS;GLYCOSYLATION AT ASN-259

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